DNA Cloning: Core Techniques / Edition 2

DNA Cloning: Core Techniques / Edition 2

by Hames Glover, David Hames, D. M. Glover
     
 

ISBN-10: 0199634769

ISBN-13: 9780199634767

Pub. Date: 03/28/2002

Publisher: Oxford University Press, USA

This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in

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Overview

This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers.

These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

Product Details

ISBN-13:
9780199634767
Publisher:
Oxford University Press, USA
Publication date:
03/28/2002
Series:
Practical Approach Series, #148
Edition description:
REV
Pages:
288
Product dimensions:
6.19(w) x 9.25(h) x 0.63(d)

Table of Contents

1. Techniques for transformation of E.coli
2. Lambda replacement vectors in the construction of genomic DNA libraries
3. Procedures for cDNA cloning
4. Making probes
5. Using cloned DNAs to analyse gene organization and expression
6. Choices of single-stranded vectors and their application for in vitro mutagenesis
7. PCR and its applications
8. DNA sequencing

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