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Doody's Review ServiceReviewer: Omer Iqbal, MD, FACC, FESC (Loyola University Medical Center)
Description: This is an excellent, beautifully illustrated resource on recent and established techniques to detect and quantify DNA damage ranging from the subcellular to the level of a whole live organism.
Purpose: The book is useful for newcomers to the field and experienced researchers. It is also intended to serve as a guide for novice molecular biology scientists to master the required techniques by providing detailed chapters with simple to follow protocols and describing possible technical pitfalls and limitations.
Audience: Novice molecular biology scientists, established researchers, and scientists from other fields, such as clinical scientists and nanobiotechnology specialists, are the intended audience. Researchers in many fields including molecular and cell biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science also will find this book quite useful. It also will be useful for graduate students interested in this field.
Features: The first of the book's three parts has seven chapters covering fixed tissue sections. It contains enzymatic approaches such as in situ detection of apoptosis by TUNEL assay and combination of TUNEL assay with immunohistochemistry for simultaneous detection of DNA fragmentation and cell damage. It also covers EM_ISEL, a useful tool to visualize DNA damage. The five chapters in the second part of the book covers detection of DNA strand damage and apoptosis in cultured cells. Nine chapters in the final part deal with methods developed either for in vivo detection, directly in a living organism, or for samples taken from the body, ex vivo — in blood, urine, and sperm. The assays often permit monitoring levels of DNA damage. The book is well illustrated with both color and black-and-white figures.
Assessment: This is an excellent resource for all major assays used in molecular and cell biology for labeling of DNA damage in situ, ex vivo, and in vivo. Graduate students, novice molecular biology scientists, and established investigators in this field will find this book of great help.