DNA Repair Protocols: Eukaryotic Systems

Overview

In DNA Repair Prools: Eukaryotic Systems, Daryl S. Henderson and a team of hands-on experts give time-tested instructions for analyzing a wide range of DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. The methods focus on eukaryotic model systems that have made, or have the potential to make, important contributions to our understanding of cellular responses to DNA damage in relation to mutagenesis, ...

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Paperback (1999)
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Overview

In DNA Repair Prools: Eukaryotic Systems, Daryl S. Henderson and a team of hands-on experts give time-tested instructions for analyzing a wide range of DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. The methods focus on eukaryotic model systems that have made, or have the potential to make, important contributions to our understanding of cellular responses to DNA damage in relation to mutagenesis, carcinogenesis, and the cell cycle. Although mammalian cells predominate, consideration is also given to such important nonmammalian model systems as yeast, C. elegans (nematode), Drosophila (fruitfly), Xenopus (amphibian), and plants.

DNA Repair Prools: Eukaryotic Systems offers the most comprehensive collection of DNA repair prools available, all step-by-step, optimized, and eminently reproducible with tips on troubleshooting and avoiding pitfalls. It will serve as the gold-standard reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches.

The book contains black-and-white illustrations.

This is a volume in the Methods in Molecular Biology series. Molecular and cell biologists, geneticists, microbiologists and biochemists will find this book of interest.

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Editorial Reviews

From the Publisher
"...It will serve as an essential reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches." - Anticancer Research
Booknews
Henderson (U. of Dundee, UK) and his team of researchers provide instructions for analyzing a wide range of DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. Topics of the 52 chapters include how to screen for DNA repair mutants in genetically accessible (mostly mammalian) model systems; how cDNAs of human repair genes can be used for phenotypic correction of repair- deficient cells; methodologies for characterizing repair proteins and for assessing levels of DNA damage and repair; methods for inducing double strand breaks in DNA and/or measuring their repair; and methods for studying "physiological" responses to DNA injury. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9781468471441
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/31/2013
  • Series: Methods in Molecular Biology Series , #113
  • Edition description: 1999
  • Pages: 641
  • Product dimensions: 5.98 (w) x 9.02 (h) x 1.34 (d)

Table of Contents

Part I. Mutant Isolation and Gene Cloning. Isolation of DNA Structure-dependent Checkpoint Mutants in S. pombe, Rui G. Martinho and Antony M. Carr. Isolating Mutants of the Nematode Caenorhabditis elegans that are Hypersensitive to DNA-damaging Agents, Phil S. Hartman and Naoaki Ishii. Isolating DNA Repair Mutants of Drosophila melanogaster, Daryl S. Henderson. Generation, Identification, and Characterization of Repair-defective Mutants of Arabidopsis, Anne Britt and Cai-Zhong Jiang. Screening for y-ray Hypersensitive Mutants of Arabidopsis, Corinne Davies. Isolation of Mutagen-sensitive Chinese Hamster Cell Lines by Replica Plating, Margaret Zdzienicka. Strategies for Cloning Mammalian DNA Repair Genes, Larry H. Thompson. Novel Complementation Assays for DNA Repair Deficient Cells: Transient and Stable Expression of DNA Repair Genes, Lin Zeng, Alain Sarasin and Mauro Mezzina. Part II. Recognition and Removal of Inappropriate or Damaged DNA Bases. The Use of Electrophoretic Mobility Shift Assays to Study DNA Repair, Byung Joon Hwang, Vaughn Smider and Gilbert Chu. Mismatch Repair Assay, Stephanie Corrette-Bennett/ Robert S. Lahue. Analysis of Cyclobutane-pyrimidine-dimer and (6-4) Photoproduct Photolyases in Crude Extracts: Chromatographic and PCR-amplifiability assays, John Hays and Peter Hoffman. A Dot Blot Immunoassay for UV Photoproducts, Shirley McCready. Measurement of Ultraviolet Radiation-induced DNA Damage Using Specific Antibodies, Ann Stapleton. Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay, David Mitchell. UV-endonuclease/ alkaline-sedimentation assay for Cyclobutane Pyrimidine Dimers in DNA from UV-irradiated Arabidopsis Plants, John B. Hays and Quishen Pang. DNA Damage Quantitation by Alkaline Gel Electrophoresis, Betsy M. Sutherland, Paula V. Bennett and John C. Sutherland. The Single Cell Gel Test (Comet Assay): A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair, G√ľnter Speit and AndreasHartmann. Measuring the Formation and Repair of UV Photoproducts by Ligation-mediated PCR, Gerd P. Pfeifer and Reinhard Dammann. PCR-based Repair Assay I., Keith Grimaldi. PCR-based Repair Assay II.,Keith Grimaldi. Gene-specific and Mihondrial Repair of Oxidative DNA Damage, Michael R. Ansan and Vilhelm A. Bohr. Characterization of DNA Strand Cleavage by Enzymes That Act at Abasic Sites in DNA, Walter Deutsch and Adly Yacoub. Base Excision repair Assay Using Xenopus laevis Oocyte Extracts, Yoshihiro Matsumoto. In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts, Guido Frosina, Enrico Cappelli, Paola Fortini, Eugenia Dogliotti. Nucleotide Excision Repair in Saccharomyces cerevisiae Whole Cell Extracts, Johnson M.S. Wong, Zhigang He and C. James Ingles. In Vitro Excision Repair Assay in Schizosaccharomyces pombe, Bernard Salles and Patrick Calsou. In Vitro Nucleotide Excision Repair Assay Using Drosophila Cell Lines, Kenji Kohno. Nucleotide Excision Repair in Nuclear Extracts from Xenopus oocytes, Eric J. Ackerman, Lilian K. Koriazova, Jitendra K. Saxena and Alexander Y. Spoonde. Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-damaged Plasmid DNA, Maureen Biggerstaff and Richard D. Wood. Dual Incision Assays for Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site. Mahmud K.K. Shivji, Jonathan G. Moggs, Isao Kuraoka and Richard D. Wood. In Vitro Chemiluminescence Assay to Measure Excision Repair in Cell Extracts, Bernard Salles and Christian Provot. Part III. DNA Strand Breakage and Repair. Physical Monitoring of HO-induced Homologous Recombination. Allyson Holmes and James E. Haber. Use of P-element Transposons to Generate Site-specific DNA Double-strand Breaks in Drosophila, Satnam S. Banga and Daryl S. Henderson. Analyzing Double-strand Repair Events in Drosophila melanogaster. Gregory Gloor, Tammy Dray and Kathy Keeler. Expression of I-Sce I in Drosophila to Induce DNA Double-stra

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