DNA Sequencing Protocols / Edition 2

by Colin A Graham, Alison J.M. Hill

Overview

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Prools, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.

Product Details

ISBN-13:	9780896037212
Publisher:	Springer-Verlag New York, LLC
Publication date:	01/10/2001
Series:	Methods in Molecular Biology , #167
Edition description:	2nd ed. 2001
Pages:	256
Product dimensions:	5.98(w) x 9.02(h) x 0.03(d)

About the Author

Graham, Colin A.; Hill, Alison J.M. (Univ of Dundee)

Table of Contents

Introduction to DNA Sequencing. The Universal Primers and the Shotgun DNA Sequencing Method. M13 Sequencing. Primer Design and Primer-Directed Sequencing. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction. Solid Phase Fluorescent Sequencing of the CFTR Gene. Shotgun DNA Sequencing. Cycle Sequencing. Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers. Automated Fluorescent DNA Sequencing on the ABI PRISM 377. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer. Fluorescent Sequencing Prools for the ALF. Fluorescent Sequencing for Heterozygote Mutation Detection. Sequence Databases and the Internet. DNA Sequencing by Capillary Array Electrophoresis. Index.

Editorial Reviews

"DNA Sequencing Prools covers the commonly used prools in a clearly written manner and is thorough in description. This book is highly recommended for all libraries serving researchers involved in any aspect of DNA sequencing." - E-STREAMS

From the Publisher

This handbook, designed for geneticists and molecular biologists, offers a variety of methods for DNA sequencing suitable for human genome sequencing and mutation detection in human disease. Step-by- step instructions and notes addressing potential problems are included for methods such as automated DNA sequencing, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases, and genome sequencing sites. Also included is a review of shotgun sequencing in the human genome project. Spiral binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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