DNA Vaccines / Edition 1

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This volume reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current applications, methods of production, and quality control. Consistent with the approach of the Methods in Molecular Biology series, these chapters contain detailed practical procedures on the latest in DNA vaccine technology.

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Editorial Reviews

Doody's Review Service
Reviewer: Rebecca T. Horvat, PhD, D (ABMM)(University of Kansas Medical Center)
Description: This new edition gives an overview of the rapid developments that have occurred in vaccine development in the seven years since the first edition. It is packed with technical and scientific information that will be helpful to scientists working on vaccines.
Purpose: In the seven years since the first edition, there have been extraordinary strides in the understanding and application of DNA vaccines. The primary purpose of this new version is to review these developments. This book shows the promise and problems associated with this new technology.
Audience: It is written for researchers in the field of vaccine development and those delving into the production of these vaccines. However, it is written clearly and is easy to follow even for a novice in the field of vaccinology. The research presented is exciting and is offered with clear explanations and rationale. The authors of the chapters clearly know their fields of investigation.
Features: This comprehensive reference takes the process of DNA vaccines from the discovery phase through the production phase. It organizes the various steps in the process into five separate sections. The first section gives several different examples of how DNA vaccines are designed. The second section reviews the techniques used to deliver the vaccines. This is followed by a section that covers the adjuvants used and the theories behind how these adjuvants enhance the activity of the vaccine. The fourth section discusses the applications for DNA vaccines and the final section reviews the production, purification, and quality of DNA vaccine production. This well organized book takes the reader from the conception of the vaccine to the production of the vaccine. It includes a treasure trove of hints, information, and procedures that would be difficult to acquire elsewhere. This is clearly the cookbook that should be used for developing DNA based vaccines.
Assessment: This book contains specific methods for the development of DNA vaccines, includes tips and ideas that have worked in various systems, and describes interesting new ideas as well as the regulatory process required for vaccine development. This book should be a staple in the library of laboratories working in this field.
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Product Details

  • ISBN-13: 9781588294845
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 8/22/2006
  • Series: Methods in Molecular Medicine Series, #127
  • Edition description: 2006
  • Edition number: 1
  • Pages: 384
  • Product dimensions: 6.14 (w) x 9.21 (h) x 0.94 (d)

Table of Contents

Part 1: DNA Vaccine Design

DNA Vaccine Design Janet Brandsma

Design of Plasmid DNA Constructs for Vaccines Donna L. Montgomery and Kristala Jones-Prather

Vaccination with Messenger RNA Steve Pascolo

A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines Petra Riedl, Nicolas Fissolo, Joerg Reimann, and Reinhold Schirmbeck

In Vitro Assay of Immunostimulatory Activities of Plasmid Vectors Weiwen Jiang, Charles Rich, and David Pisetsky

Part 2: DNA Vaccine Delivery Systems

Delivery of DNA Vaccines Using Electroporation Shawn Babiuk, Sylvia van Drunen Little-van den Hurk, and Lorne Babiuk

Needle-Free Injection of DNA Vaccines Kanakatte Raviprakash and Kevin Porter

Needle-Free Delivery of Veterinary DNA Vaccines Sylvia van Drunen Little-van den Hurk, Shawn Babiuk, and Lorne Babiuk

Surface-Modified Biodegradable Microspheres for DNA Vaccine Delivery Mark Keegan and Mark Saltzman

A Dendrimer-Like DNA-Based Vector for DNA Delivery: A Viral and Non-Viral Hybrid Approach Dan Luo, Yougen Li, Soong Ho Um, and Yen Cu

Identification of Compartments Involved in Mammalian Subcellular Trafficking Pathways by Indirect Immunofluorescence Anne Doody and David Putnam

Part 3: DNA Vaccine Adjuvants and Activity Enhancement

Adjuvant Properties of CpG Oligonucleotides in Primates Daniela Verthelyi

Complexes of DNA Vaccines with Cationic, Antigenic Peptides are Potent, Polyvalent CD8+ T Cell-Stimulating Agents Petra Riedl, Joerg Reimann, and Reinhold Schirmbeck

Prime Boost Strategies in DNA Vaccines C. Jane Dale, Scott Thomson, Robert De Rose, Charani Ranasinghe, C. Jill Medveczky, Joko Pamungkas, David B. Boyle, Ian A. Ramshaw, and Stephen J. Kent

Modifying Professional Antigen Presenting Cells to Enhance DNA Vaccine Potency C.-F. Hung, M. Yang, and T.C. Wu

Replicase-Based DNA Vaccines for Allergy Treatment Sandra Scheiblhofer, Richard Weiss, Maximilian Gabler, Wolfgang Leitner, and Josef Thalhamer

Part 4: DNA Vaccine Applications

Immunological Responses of Neonates and Infants to DNA Vaccines Martha Sedegah and Stephen L. Hoffman

DNA Vaccines for Allergy Treatment Richard Weiss, Sandra Scheiblhofer, and Josef Thalhamer

Protection From Autoimmunity by DNA Vaccination Against T Cell Receptor (TCR)
Thorsten Buch and Ari Waisman

Use of Bone Marrow-Chimeric Mice in Elucidating Immune Mechanisms Akiko Iwasaki

Part 5: DNA Vaccine Production, Purification, and Quality

A Simple Method for the Production of Plasmid DNA in Bioreactors K. Listner, L. Kiser Bentley, and Michael Chartrain

Practical Methods for Supercoiled pDNA Production John Ballantyne

Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelines Joachim Schorr, Peter Moritz, Astrid Breul, and Martin Scheef

Large-Scale Non-Chromatographic Purification of Plasmid DNA Jason Murphy and Sangeetha Sagar

Assuring the Quality, Safety and Efficacy of DNA Vaccines James S. Robertson

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