DNA Vaccines / Edition 1

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Overview

In this completely revised and updated second edition of DNA Vaccines: Methods and Prools, W. Mark Saltzman presents a comprehensive collection of DNA vaccine prools, written by leading experts in the groundbreaking field of DNA vaccination. Divided into five sections, this volume contains state-of-the-art and practical procedures on the latest DNA vaccine technology. Part I contains DNA vaccine design prools, focusing on methods that achieve optimal expression in host cells. Part II is dedicated to presenting methods for DNA delivery, and covers both the range of administration methods available for vaccine administration and a variety of techniques for improving the efficiency of delivery into cells. Part III discusses current available methods, including adjuvant and prime-boost approaches, for enhancing the potency of DNA vaccines. Part IV describes several key areas of application in the field, including allergy, avoidance of autoimmunity, and neonate and infant vaccine response. DNA Vaccines: Methods and Prools, Second Edition concludes with a review of prools for vaccine production and purification, and applicable quality control methods.

This collection of up-to-date prools demonstrates the tremendous progress that has been made in the field of DNA vaccination since the initial appearance of this new vaccination strategy. Given current enthusiasm for the magnificent potential for this technology among the scientific community, researchers will find tremendous value in this updated and enhanced molecular methods guide.

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Editorial Reviews

Doody's Review Service
Reviewer: Rebecca T. Horvat, PhD, D (ABMM)(University of Kansas Medical Center)
Description: This new edition gives an overview of the rapid developments that have occurred in vaccine development in the seven years since the first edition. It is packed with technical and scientific information that will be helpful to scientists working on vaccines.
Purpose: In the seven years since the first edition, there have been extraordinary strides in the understanding and application of DNA vaccines. The primary purpose of this new version is to review these developments. This book shows the promise and problems associated with this new technology.
Audience: It is written for researchers in the field of vaccine development and those delving into the production of these vaccines. However, it is written clearly and is easy to follow even for a novice in the field of vaccinology. The research presented is exciting and is offered with clear explanations and rationale. The authors of the chapters clearly know their fields of investigation.
Features: This comprehensive reference takes the process of DNA vaccines from the discovery phase through the production phase. It organizes the various steps in the process into five separate sections. The first section gives several different examples of how DNA vaccines are designed. The second section reviews the techniques used to deliver the vaccines. This is followed by a section that covers the adjuvants used and the theories behind how these adjuvants enhance the activity of the vaccine. The fourth section discusses the applications for DNA vaccines and the final section reviews the production, purification, and quality of DNA vaccine production. This well organized book takes the reader from the conception of the vaccine to the production of the vaccine. It includes a treasure trove of hints, information, and procedures that would be difficult to acquire elsewhere. This is clearly the cookbook that should be used for developing DNA based vaccines.
Assessment: This book contains specific methods for the development of DNA vaccines, includes tips and ideas that have worked in various systems, and describes interesting new ideas as well as the regulatory process required for vaccine development. This book should be a staple in the library of laboratories working in this field.
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Product Details

  • ISBN-13: 9781617375965
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/10/2010
  • Series: Methods in Molecular Medicine Series , #127
  • Edition description: Softcover reprint of hardcover 1st ed. 2006
  • Edition number: 1
  • Pages: 384
  • Product dimensions: 0.89 (w) x 6.00 (h) x 9.00 (d)

Table of Contents

Part 1: DNA Vaccine Design

DNA Vaccine Design
Janet Brandsma

Design of Plasmid DNA Constructs for Vaccines
Donna L. Montgomery and Kristala Jones-Prather

Vaccination with Messenger RNA
Steve Pascolo

A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines
Petra Riedl, Nicolas Fissolo, Joerg Reimann, and Reinhold Schirmbeck

In Vitro Assay of Immunostimulatory Activities of Plasmid Vectors
Weiwen Jiang, Charles Rich, and David Pisetsky

Part 2: DNA Vaccine Delivery Systems

Delivery of DNA Vaccines Using Electroporation
Shawn Babiuk, Sylvia van Drunen Little-van den Hurk, and Lorne Babiuk

Needle-Free Injection of DNA Vaccines
Kanakatte Raviprakash and Kevin Porter

Needle-Free Delivery of Veterinary DNA Vaccines
Sylvia van Drunen Little-van den Hurk, Shawn Babiuk, and Lorne Babiuk

Surface-Modified Biodegradable Microspheres for DNA Vaccine Delivery
Mark Keegan and Mark Saltzman

A Dendrimer-Like DNA-Based Vector for DNA Delivery: A Viral and Non-Viral Hybrid Approach
Dan Luo, Yougen Li, Soong Ho Um, and Yen Cu

Identification of Compartments Involved in Mammalian Subcellular Trafficking Pathways by Indirect Immunofluorescence
Anne Doody and David Putnam

Part 3: DNA Vaccine Adjuvants and Activity Enhancement

Adjuvant Properties of CpG Oligonucleotides in Primates
Daniela Verthelyi

Complexes of DNA Vaccines with Cationic, Antigenic Peptides are Potent, Polyvalent CD8+ T Cell-Stimulating Agents
Petra Riedl, Joerg Reimann, and Reinhold Schirmbeck

Prime Boost Strategies in DNA Vaccines
C. Jane Dale, Scott Thomson, Robert De Rose, Charani Ranasinghe, C. Jill Medveczky, Joko Pamungkas, David B. Boyle, Ian A. Ramshaw, and Stephen J. Kent

Modifying Professional Antigen Presenting Cells to Enhance DNA Vaccine Potency
C.-F. Hung, M. Yang, and T.C. Wu

Replicase-Based DNA Vaccines for Allergy Treatment
Sandra Scheiblhofer, Richard Weiss, Maximilian Gabler, Wolfgang Leitner, and Josef Thalhamer

Part 4: DNA Vaccine Applications

Immunological Responses of Neonates and Infants to DNA Vaccines
Martha Sedegah and Stephen L. Hoffman

DNA Vaccines for Allergy Treatment
Richard Weiss, Sandra Scheiblhofer, and Josef Thalhamer

Protection From Autoimmunity by DNA Vaccination Against T Cell Receptor (TCR)
Thorsten Buch and Ari Waisman

Use of Bone Marrow-Chimeric Mice in Elucidating Immune Mechanisms
Akiko Iwasaki

Part 5: DNA Vaccine Production, Purification, and Quality

A Simple Method for the Production of Plasmid DNA in Bioreactors
K. Listner, L. Kiser Bentley, and Michael Chartrain

Practical Methods for Supercoiled pDNA Production
John Ballantyne

Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelines
Joachim Schorr, Peter Moritz, Astrid Breul, and Martin Scheef

Large-Scale Non-Chromatographic Purification of Plasmid DNA
Jason Murphy and Sangeetha Sagar

Assuring the Quality, Safety and Efficacy of DNA Vaccines
James S. Robertson

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