DNA Viruses: A Practical Approach / Edition 1

DNA Viruses: A Practical Approach / Edition 1

by Alan J. Cann
     
 

ISBN-10: 0199637180

ISBN-13: 9780199637188

Pub. Date: 02/28/2000

Publisher: Oxford University Press, USA

DNA Viruses: A Practical Approach groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinical isolates. Chapter 1 provides an overview of the extraction,

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Overview

DNA Viruses: A Practical Approach groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinical isolates. Chapter 1 provides an overview of the extraction, purification and characterizations of virus DNA, but also covers the fundamentals of DNA virus culture. Chapters 2 and 3 describe approaches to the molecular investigation and mutagenesis of DNA virus genomes. Chapter 4 considers DNA virus replication and then chapters 5 & 6 describe methods to study transcription control. Chapters 7 to 9 consider aspects of the pathogenesis of DNA virus infections. The final chapter describes the current technology being applied to the development of DNA virus vectors for gene delivery. This volume will therefore be of interest to all those working on DNA viruses whether in academia, industry or clinical research.

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Product Details

ISBN-13:
9780199637188
Publisher:
Oxford University Press, USA
Publication date:
02/28/2000
Series:
Practical Approach Series, #214
Edition description:
New Edition
Pages:
336
Product dimensions:
6.10(w) x 9.10(h) x 0.60(d)

Table of Contents

List of Contributors
Abbreviations
1. Extraction, purification, and characterization of virus DNA, Ian W. Halliburton
2. Investigation of DNA virus genome structure, Michael A. Skinner and Stephen M. Laidlaw
3. Mutagenesis of DNA virus genomes, Keith N. Leppard
4. Interactions between viral and cellular proteins during DNA virus replication, Catherine H. Botting and Ronald T. Hay
5. Analysis of transcriptional control in DNA virus infections, S. K. Thomas and D. S. Latchman
6. Identification and analysis of trans-acting proteins involved in the regulation of DNA virus gene expression, Adrian Whitehouse and David M. Meredith
7. Interaction of DNA virus proteins with host cytokines, Alshad S. Lalani et al.
8. Analysis of DNA virus proteins involved in neoplastic cell transformation, Kersten T. Hall, Maria E. Blair Zajdel, and G. Eric Blair
9. Chemotherapy of DNA virus infections, Patricia A. Cane and Deenan Pillay
10. Herpes simplex virus and adenovirus vectors, Cinzia Scarpini et al.
Appendix
Index

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