DNA Viruses: A Practical Approach / Edition 1

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Overview


DNA Viruses: A Practical Approach groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinical isolates. Chapter 1 provides an overview of the extraction, purification and characterizations of virus DNA, but also covers the fundamentals of DNA virus culture. Chapters 2 and 3 describe approaches to the molecular investigation and mutagenesis of DNA virus genomes. Chapter 4 considers DNA virus replication and then chapters 5 & 6 describe methods to study transcription control. Chapters 7 to 9 consider aspects of the pathogenesis of DNA virus infections. The final chapter describes the current technology being applied to the development of DNA virus vectors for gene delivery. This volume will be of interest to all those working on DNA viruses whether in academia, industry or clinical research.

The book contains predominantly black-and-white illustrations, with some color illustrations.

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Editorial Reviews

Doody's Review Service
Reviewer: Jerry Lynn Taylor, PhD (Medical College of Wisconsin)
Description: This book contains detailed protocols ranging from basic to more sophisticated methods for working with DNA viruses. Each protocol is presented as a stepwise, concise procedure. Explanations of the rationale for each procedure and potential problems in performance of the procedure or interpretation of the data are discussed.
Purpose: The purpose is to compile in a single volume descriptions of many of the techniques used by virologists to study DNA viruses. This objective is clearly met. The detailed protocols were developed by virologists who actually use the methods in their own research.
Audience: This book was written by well-respected virologists for virologists and students of virology. However, as the editor states, often methods developed by virologists have proven to be applicable for the study of many aspects of host cell biology as well.
Features: Very basic methods are covered, such as growth and purification of viruses to more complex procedures including many that focus on examining host/virus interactions (for example, how viruses regulate cytokine function). In addition to the stepwise protocols, contributors explain what kind of data can be expected from the protocol and how to interpret that data. Different DNA viruses serve as examples for each protocol. References to original articles are provided so that the reader can go to the literature to see applications of the procedures.
Assessment: This book would be a good practical handbook for students of virology. Although the focus is on DNA viruses, many of the methods are also applicable to the study of RNA viruses and cell biology.
Jerry Lynn Taylor
This book contains detailed protocols ranging from basic to more sophisticated methods for working with DNA viruses. Each protocol is presented as a stepwise, concise procedure. Explanations of the rationale for each procedure and potential problems in performance of the procedure or interpretation of the data are discussed. The purpose is to compile in a single volume descriptions of many of the techniques used by virologists to study DNA viruses. This objective is clearly met. The detailed protocols were developed by virologists who actually use the methods in their own research. This book was written by well-respected virologists for virologists and students of virology. However, as the editor states, often methods developed by virologists have proven to be applicable for the study of many aspects of host cell biology as well. Very basic methods are covered, such as growth and purification of viruses to more complex procedures including many that focus on examining host/virus interactions (for example, how viruses regulate cytokine function). In addition to the stepwise protocols, contributors explain what kind of data can be expected from the protocol and how to interpret that data. Different DNA viruses serve as examples for each protocol. References to original articles are provided so that the reader can go to the literature to see applications of the procedures. This book would be a good practical handbook for students of virology. Although the focus is on DNA viruses, many of the methods are also applicable to the study of RNA viruses and cell biology.
Booknews
Describes in detail major experimental methods for studying DNA viruses, from fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and real-time PCR analysis of drug resistance mutations. Describes the culture of DNA viruses and the extraction, purification, and characterization of virus DNA, then discusses aspects of virus genome mapping, correlation of genotype with phenotype, sequence analysis, mutagenesis of DNA virus genomes, and identification and study of trans-acting regulatory proteins. Cann teaches in the department of microbiology and immunology at the University of Leicester. Annotation c. Book News, Inc., Portland, OR (booknews.com)

4 Stars! from Doody
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Product Details

  • ISBN-13: 9780199637188
  • Publisher: Oxford University Press, USA
  • Publication date: 2/28/2000
  • Series: Practical Approach Series , #214
  • Edition description: New Edition
  • Edition number: 1
  • Pages: 336
  • Product dimensions: 6.10 (w) x 9.10 (h) x 0.60 (d)

Table of Contents

List of Contributors
Abbreviations
1. Extraction, purification, and characterization of virus DNA, Ian W. Halliburton
2. Investigation of DNA virus genome structure, Michael A. Skinner and Stephen M. Laidlaw
3. Mutagenesis of DNA virus genomes, Keith N. Leppard
4. Interactions between viral and cellular proteins during DNA virus replication, Catherine H. Botting and Ronald T. Hay
5. Analysis of transcriptional control in DNA virus infections, S. K. Thomas and D. S. Latchman
6. Identification and analysis of trans-acting proteins involved in the regulation of DNA virus gene expression, Adrian Whitehouse and David M. Meredith
7. Interaction of DNA virus proteins with host cytokines, Alshad S. Lalani et al.
8. Analysis of DNA virus proteins involved in neoplastic cell transformation, Kersten T. Hall, Maria E. Blair Zajdel, and G. Eric Blair
9. Chemotherapy of DNA virus infections, Patricia A. Cane and Deenan Pillay
10. Herpes simplex virus and adenovirus vectors, Cinzia Scarpini et al.
Appendix
Index

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