DNA Viruses: Methods and Protocols / Edition 1

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A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Prools will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.

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Editorial Reviews

Doody's Review Service
Reviewer: Eugene A Davidson, PhD (Georgetown University School of Medicine)
Description: This book contains a set of protocols for laboratory work with DNA viruses. Detection, imaging, and basic molecular biology are covered.
Purpose: The goal is to provide laboratory personnel with protocols for working with DNA viruses. Given the medical importance of many of these, this is a worthy end. Objectives are broadly fulfilled but with limitations and some omissions.
Audience: Individuals who will use this book include advanced students and laboratory personnel. The contributors are generally recognized investigators.
Features: This is a compilation of protocols designed to provide laboratory directions for scientists working with DNA viruses. The material is divided into several sections and includes coverage of virus preparation, in situ imaging, interaction with cellular targets, and general aspects of viral molecular biology including methods for controlling expression of specific genes. Each of the protocols is presented with sufficient experimental detail to allow ready use by most laboratory personnel. Accompanying bibliographies provide reference to primary sources should these be desired. Many of the examples are taken from work with a selected subset — herpes and papilloma. Although all of the material is clearly presented, there are limitations. This class of viruses is highly important in several medical areas including gene therapy and vaccine development — thus, a cervical cancer vaccine based on papillomavirus is entering clinical trials. Also absent is any discussion of the potential hazards associated with the use of one or more of these agents in biowarfare — smallpox is the most trenchant candidate. Most laboratories working with DNA viruses will find ample useful material here.
Assessment: Good presentation and reasonable coverage mark this book, but some timely topics are missing. It is a workable laboratory manual.

3 Stars from Doody
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Product Details

  • ISBN-13: 9781588293534
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 10/28/2004
  • Series: Methods in Molecular Biology Series, #292
  • Edition description: 2005
  • Edition number: 1
  • Pages: 512
  • Product dimensions: 9.10 (w) x 6.20 (h) x 1.10 (d)

Table of Contents

Part I. Viral Detection

Viral Detection
Feng Wang-Johanning and Gary L. Johanning

Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1
Servi J. C. Stevens, Sandra A. W. M. Verkuijlen, and Jaap M. Middeldorp

Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCR
Servi J. C. Stevens, Antoinette A. T. P. Brink, and Jaap M. Middeldorp

Quantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells In Vivo
Donna R. Hochberg and David A. Thorley-Lawson

Detection and Quantification of the Rare Latently Infected Cell Undergoing Herpes Simplex Virus Transcriptional Activation in the Nervous System In Vivo
Nancy M. Sawtell

Reporter Cell Lines for the Detection of Herpes Simplex Viruses
Szu-Hao Kung

Part II. Virus Structure and Imaging

Unraveling the Architecture of Viruses by High-Resolution Atomic Force Microscopy
Alexander J. Malkin, Marco Plomp, and Alexander McPherson

Studying the Structure of Large Viruses With Multiresolution Imaging
Carmen San Martín

Herpes Simplex Virus-Cell Interactions Studied by Low-Fading Contrasted Immunofluorescence
Helle Lone Jensen and Bodil Norrild

Herpes Simplex Virus-Cell Interactions Studied by Immunogold Cryosection Electron Microscopy
Helle Lone Jensen and Bodil Norrild

FTIR Microscopy Detection of Cells Infected With Viruses
Vitaly Erukhimovitch, Marina Talyshinsky, Yelena Souprun, and Mahmoud Huleihel

Part III. Virus Entry

The JC Virus-Like Particle Overlay Assay
Hirofumi Sawa and Rika Komagome

Analysis of Fusion Using a Virus-Free Cell Fusion Assay
Marisa P. McShane and Richard Longnecker

Pseudovirions asSpecific Tools for Investigation of Virus Interactions With Cells
Martin Sapp and Hans-Christoph Selinka

Part IV. Gene Expression

Simultaneous In Situ Detection of RNA, DNA, and Protein Using Tyramide-Coupled Immunofluorescence
Brian A. Van Tine, Thomas R. Broker, and Louise T. Chow

Identification and Characterization of Herpesviral Immediate-Early Genes
Yan Yuan

Part V. Replication and Genome Maintenance

Methods for Measuring the Replication and Segregation of Epstein-Barr Virus-Based Plasmids
Priya Kapoor and Lori Frappier

DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins
Constandache Atanasiu, Larissa Lezina, and Paul M. Lieberman

Part VI. Pathogenesis

Pre-B-Cell Colony Formation Assay
Masato Ikeda and Richard Longnecker

Luciferase Real-Time Bioluminescence Imaging for the Study of Viral Pathogenesis
Gary D. Luker and David A. Leib

Part VII. Complex Cell Systems

Culturing Primary and Transformed Neuronal Cells for Studying Pseudorabies Virus Infection
Toh Hean Ch'ng, E. Alexander Flood, and Lynn William Enquist

Human Papillomavirus Type 31 Life Cycle: Methods for Study Using Tissue Culture Models
Frauke Fehrmann and Laimonis A. Laimins

Part VIII. Recombinant Genetics

Molecular Genetics of Herpesviruses: A Recombinant Technology Approach
Jason S. Knight, Subhash C. Verma, Ke Lan, and Erle S. Robertson

Molecular Genetics of DNA Viruses: Recombinant Virus Technology
Bernhard Neuhierl and Henri-Jacques Delecluse

Genetic Analysis of Cytomegalovirus by Shuttle Mutagenesis
Manfred Lee and Fenyong Liu

Construction of a Gene Inactivation Library for Bovine herpesvirus 1 Using Infectious Clone Technology
Timothy J. Mahony, Fiona M. McCarthy, Jennifer L. Gravel, and Peter L. Young

Selective Sile

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