Doubled Haploid Production in Crop Plants: A Manual / Edition 1

Doubled Haploid Production in Crop Plants: A Manual / Edition 1

by M. Maluszynski
     
 

ISBN-10: 1402015445

ISBN-13: 9781402015441

Pub. Date: 11/30/2003

Publisher: Springer Netherlands

This is the first manual presenting a set of protocols for production of doubled haploids (DH) in 22 major crop plant species including 4 tree species. It contains various protocols and approaches of DH production proven for different germplasm of the same species. The protocols describe in detail all steps of DH production - from donor plants growth conditions,

Overview

This is the first manual presenting a set of protocols for production of doubled haploids (DH) in 22 major crop plant species including 4 tree species. It contains various protocols and approaches of DH production proven for different germplasm of the same species. The protocols describe in detail all steps of DH production - from donor plants growth conditions, through in vitro procedures, media composition and preparation, to regeneration of haploid plants and chromosome doubling methods. The users of this manual will be able to choose among microspore versus anther cultures, wide hybridisation or gynogenesis, the most suitable method for production of DH in particular laboratory conditions for their plant material. Numerous colour illustrations should help in this decision. The user will also find information on organization of a doubled haploid laboratory, basic DH media and on associated simple cytogenetic methods for ploidy level analysis. The practical protocols are supplemented with the list of published DH protocols for other crop plants and separate chapters dealing with major applications of DH in breeding, mutant production, transgenesis, and genetic mapping and genomics. The book was prepared by the Plant Breeding and Genetics Section of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture in close co-operation with the EU program 'COST 851' on 'Gametic cells and molecular breeding for crop improvement'.

Product Details

ISBN-13:
9781402015441
Publisher:
Springer Netherlands
Publication date:
11/30/2003
Edition description:
2003
Pages:
428
Product dimensions:
6.10(w) x 9.25(h) x 0.05(d)

Related Subjects

Table of Contents

Prefacevii
Abbreviationsix
Colour Figuresxi
1.Production of doubled haploids in crop plants. An introduction1
2.Protocols for major crops
Barley
2.1.Doubled haploid production in barley using the Hordeum bulbosum (L.) technique5
2.2.The Hordeum bulbosum (L.) method15
2.3.Anther culture in barley21
2.4.Barley anther culture29
2.5.Anther culture for doubled haploid production in barley (Hordeum vulgare L.)35
2.6.Barley isolated microspore culture protocol43
2.7.Barley isolated microspore culture (IMC) method49
Wheat
2.8.Doubled haploid production in wheat through wide hybridization53
2.9.Protocol of wheat (Triticum eastivum L.) anther culture59
2.10.Protocol for producing doubled haploid plants from anther culture of wheat (Triticum aestivum L.)65
2.11.Wheat anther culture71
2.12.Haploid wheat isolated microspore culture protocol77
2.13.Production of doubled haploids in wheat (Triticum aestivum L.) through microspore embryogenesis triggered by inducer chemicals83
Maize
2.14.Isolated microspore culture in maize (Zea mays L.), production of doubled-haploid via induced androgenesis95
2.15.Anther culture of maize (Zea mays L.)103
Rice indica/japanica
2.16.Laboratory protocol for anther culture technique in rice109
Triticale
2.17.Triticale anther culture117
2.18.Protocol for anther culture in hexaploid triticale (x Triticosecale Wittm.)123
2.19.Protocol of triticale (x Triticosecale Wittmack) microspore culture129
2.20.Protocol for doubled haploid production in hexaploid triticale (x Triticosecale Wittm.) by crosses with maize135
Rye
2.21.Protocol for rye anther culture141
2.22.Microspore culture of rye151
Oat
2.23.Oat haploids from wide hybridization155
Durum wheat
2.24.Haploid and doubled haploid production in durum wheat by wide hybridization161
2.25.Haploid and doubled haploid production in durum wheat by anther culture167
Timothy
2.26.Anther culture and isolated microspore culture in timothy173
Ryegrass and other grasses
2.27.Doubled haploid induction in ryegrass and other grasses179
Rapeseed
2.28.Microspore culture in rapeseed (Brassica napus L.)185
Broccoli
2.29.Protocol for broccoli microspore culture195
Other Brassicas
2.30.Microspore culture of Brassica species205
2.31.Protocol for microspore culture in Brassica217
Tobacco
2.32.Anther and microspore culture in tobacco223
Potato
2.33.Haploid production of potatoes by anther culture229
2.34.Anther culture through direct embryogenesis in a genetically diverse range of potato (Solanum) species and their interspecific and intergeneric hybrids235
2.35.Potato haploid technologies241
Linseed/flax
2.36.Anther culture of linseed (Linum usitatissimum L.)249
Sugar beet
2.37.Doubled haploid production of sugar beet (Beta vulgaris L.)255
Asparagus
2.38.Asparagus microspore and anther culture265
Onion
2.39.In vitro gynogenesis induction and doubled haploid production in onion (Allium cepa L.)275
2.40.Haploid induction in onion via gynogenesis281
Apple
2.41.In vitro androgenesis in apple287
Aspen
2.42.Doubled haploid production in poplar293
Cork oak
2.43.Oak anther culture297
Citrus
2.44.Haploids and doubled haploids in Citrus spp.303
3.Published protocols for other crop plant species309
4.Application of doubled haploid production techniques
4.1.Doubled haploids in breeding337
4.2.Doubled haploid mutant production351
4.3.Barley microspore transformation protocol by biolistic gun363
4.4.Doubled haploids in genetic mapping and genomics367
5.Protocols for chromosome counting
5.1.Cytogenetic tests for ploidy level analyses--chromosome counting391
5.2.Ploidy determination using flow cytometry397
6.Major media composition and basic equipment for DH laboratory
6.1.Major media composition405
6.2.Basic equipment for maize microspore culture laboratory415
List of contributors417

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