Doubled Haploid Production in Crop Plants: A Manual / Edition 1

Doubled Haploid Production in Crop Plants: A Manual / Edition 1

by M. Maluszynski
     
 

This is the first manual presenting a set of protocols for production of doubled haploids (DH) in 22 major crop plant species including 4 tree species. It contains various protocols and approaches of DH production proven for different germplasm of the same species. The protocols describe in detail all steps of DH production - from donor plants growth conditions,

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Overview

This is the first manual presenting a set of protocols for production of doubled haploids (DH) in 22 major crop plant species including 4 tree species. It contains various protocols and approaches of DH production proven for different germplasm of the same species. The protocols describe in detail all steps of DH production - from donor plants growth conditions, through in vitro procedures, media composition and preparation, to regeneration of haploid plants and chromosome doubling methods. The users of this manual will be able to choose among microspore versus anther cultures, wide hybridisation or gynogenesis, the most suitable method for production of DH in particular laboratory conditions for their plant material. Numerous colour illustrations should help in this decision. The user will also find information on organization of a doubled haploid laboratory, basic DH media and on associated simple cytogenetic methods for ploidy level analysis. The practical protocols are supplemented with the list of published DH protocols for other crop plants and separate chapters dealing with major applications of DH in breeding, mutant production, transgenesis, and genetic mapping and genomics. The book was prepared by the Plant Breeding and Genetics Section of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture in close co-operation with the EU program 'COST 851' on 'Gametic cells and molecular breeding for crop improvement'.

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Product Details

ISBN-13:
9781402015441
Publisher:
Springer Netherlands
Publication date:
11/28/2003
Edition description:
2003
Pages:
428
Product dimensions:
6.40(w) x 9.50(h) x 1.30(d)

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Table of Contents

Preface
Abbreviations
Colour Figures
1Production of doubled haploids in crop plants. An introduction1
2Protocols for major crops
2.1Doubled haploid production in barley using the Hordeum bulbosum (L.) technique5
2.2The Hordeum bulbosum (L.) Method15
2.3Anther culture in barley21
2.4Barley anther culture29
2.5Anther culture for doubled haploid production in barley (Hordeum vulgare L.)35
2.6Barley isolated microspore culture protocol43
2.7Barley isolated microspore culture (IMC) method49
2.8Doubled haploid production in wheat through wide hybridization53
2.9Protocol of wheat (Triticum eastivum L.) anther culture59
2.10Protocol for producing doubled haploid plants from anther culture of wheat (Triticum aestivum L.)65
2.11Wheat anther culture71
2.12Haploid wheat isolated microspore culture protocol77
2.13Production of doubled haploids in wheat (Triticum aestivum L.) through microspore embryogenesis triggered by inducer chemicals83
2.14Isolated microspore culture in maize (Zea mays L.), production of doubled-haploid via induced androgenesis95
2.15Anther culture of maize (Zea mays L.)103
2.16Laboratory protocol for anther culture technique in rice109
2.17Triticale anther culture117
2.18Protocol for anther culture in hexaploid triticale (x Triticosecale Wittm)123
2.19Protocol of triticale (x Triticosecale Wittmack) microspore culture129
2.20Protocol for doubled haploid production in hexaploid triticale (x Triticosecale Wittm.) by crosses with maize135
2.21Protocol for rye anther culture141
2.22Microspore culture of rye151
2.23Oat haploids from wide hybridization155
2.24Haploid and doubled haploid production in durum wheat by wide hybridization161
2.25Haploid and doubled haploid production in durum wheat by anther culture167
2.26Anther culture and isolated microspore culture in timothy173
2.27Doubled haploid induction in ryegrass and other grasses179
2.28Microspore culture in rapeseed (Brassica napus L.)185
2.29Protocol for broccoli microspore culture195
2.30Microspore culture of Brassica species205
2.31Protocol for microspore culture in Brassica217
2.32Anther and microspore culture in tobacco223
2.33Haploid production of potatoes by anther culture229
2.34Anther culture through direct embryogenesis in a genetically diverse range of potato (Solanum) species and their interspecific and intergeneric hybrids235
2.35Potato haploid technologies241
2.36Anther culture of linseed (Linum usitatissimum L.)249
2.37Doubled haploid production of sugar beet (Beta vulgaris L.)255
2.38Asparagus microspore and anther culture265
2.39In vitro gynogenesis induction and doubled haploid production in onion (Allium cepa L.)275
2.40Haploid induction in onion via gynogenesis281
2.41In vitro androgenesis in apple287
2.42Doubled haploid production in poplar293
2.43Oak anther culture297
2.44Haploids and doubled haploids in Citrus spp303
3Published protocols for other crop plant species309
4Application of doubled haploid production techniques
4.1Doubled haploids in breeding337
4.2Doubled haploid mutant production351
4.3Barley microspore transformation protocol by biolistic gun363
4.4Doubled haploids in genetic mapping and genomics367
5Protocols for chromosome counting
5.1Cytogenetic tests for ploidy level analyses - chromosome counting391
5.2Ploidy determination using flow cytometry397
6Major media composition and basic equipment for DH laboratory
6.1Major media composition405
6.2Basic equipment for maize microspore culture laboratory415
List of contributors417

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