Doubled Haploid Production in Crop Plants: A Manual / Edition 1

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This is the first manual presenting a set of protocols for production of doubled haploids (DH) in 22 major crop plant species including 4 tree species. It contains various protocols and approaches of DH production proven for different germplasm of the same species. The protocols describe in detail all steps of DH production - from donor plants growth conditions, through in vitro procedures, media composition and preparation, to regeneration of haploid plants and chromosome doubling methods. The users of this manual will be able to choose among microspore versus anther cultures, wide hybridisation or gynogenesis, the most suitable method for production of DH in particular laboratory conditions for their plant material. Numerous colour illustrations should help in this decision. The user will also find information on organization of a doubled haploid laboratory, basic DH media and on associated simple cytogenetic methods for ploidy level analysis. The practical protocols are supplemented with the list of published DH protocols for other crop plants and separate chapters dealing with major applications of DH in breeding, mutant production, transgenesis, and genetic mapping and genomics. The book was prepared by the Plant Breeding and Genetics Section of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture in close co-operation with the EU program 'COST 851' on 'Gametic cells and molecular breeding for crop improvement'.

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Product Details

  • ISBN-13: 9781402015441
  • Publisher: Springer Netherlands
  • Publication date: 11/28/2003
  • Edition description: 2003
  • Edition number: 1
  • Pages: 428
  • Product dimensions: 6.40 (w) x 9.50 (h) x 1.30 (d)

Table of Contents

Colour Figures
1 Production of doubled haploids in crop plants. An introduction 1
2 Protocols for major crops
2.1 Doubled haploid production in barley using the Hordeum bulbosum (L.) technique 5
2.2 The Hordeum bulbosum (L.) Method 15
2.3 Anther culture in barley 21
2.4 Barley anther culture 29
2.5 Anther culture for doubled haploid production in barley (Hordeum vulgare L.) 35
2.6 Barley isolated microspore culture protocol 43
2.7 Barley isolated microspore culture (IMC) method 49
2.8 Doubled haploid production in wheat through wide hybridization 53
2.9 Protocol of wheat (Triticum eastivum L.) anther culture 59
2.10 Protocol for producing doubled haploid plants from anther culture of wheat (Triticum aestivum L.) 65
2.11 Wheat anther culture 71
2.12 Haploid wheat isolated microspore culture protocol 77
2.13 Production of doubled haploids in wheat (Triticum aestivum L.) through microspore embryogenesis triggered by inducer chemicals 83
2.14 Isolated microspore culture in maize (Zea mays L.), production of doubled-haploid via induced androgenesis 95
2.15 Anther culture of maize (Zea mays L.) 103
2.16 Laboratory protocol for anther culture technique in rice 109
2.17 Triticale anther culture 117
2.18 Protocol for anther culture in hexaploid triticale (x Triticosecale Wittm) 123
2.19 Protocol of triticale (x Triticosecale Wittmack) microspore culture 129
2.20 Protocol for doubled haploid production in hexaploid triticale (x Triticosecale Wittm.) by crosses with maize 135
2.21 Protocol for rye anther culture 141
2.22 Microspore culture of rye 151
2.23 Oat haploids from wide hybridization 155
2.24 Haploid and doubled haploid production in durum wheat by wide hybridization 161
2.25 Haploid and doubled haploid production in durum wheat by anther culture 167
2.26 Anther culture and isolated microspore culture in timothy 173
2.27 Doubled haploid induction in ryegrass and other grasses 179
2.28 Microspore culture in rapeseed (Brassica napus L.) 185
2.29 Protocol for broccoli microspore culture 195
2.30 Microspore culture of Brassica species 205
2.31 Protocol for microspore culture in Brassica 217
2.32 Anther and microspore culture in tobacco 223
2.33 Haploid production of potatoes by anther culture 229
2.34 Anther culture through direct embryogenesis in a genetically diverse range of potato (Solanum) species and their interspecific and intergeneric hybrids 235
2.35 Potato haploid technologies 241
2.36 Anther culture of linseed (Linum usitatissimum L.) 249
2.37 Doubled haploid production of sugar beet (Beta vulgaris L.) 255
2.38 Asparagus microspore and anther culture 265
2.39 In vitro gynogenesis induction and doubled haploid production in onion (Allium cepa L.) 275
2.40 Haploid induction in onion via gynogenesis 281
2.41 In vitro androgenesis in apple 287
2.42 Doubled haploid production in poplar 293
2.43 Oak anther culture 297
2.44 Haploids and doubled haploids in Citrus spp 303
3 Published protocols for other crop plant species 309
4 Application of doubled haploid production techniques
4.1 Doubled haploids in breeding 337
4.2 Doubled haploid mutant production 351
4.3 Barley microspore transformation protocol by biolistic gun 363
4.4 Doubled haploids in genetic mapping and genomics 367
5 Protocols for chromosome counting
5.1 Cytogenetic tests for ploidy level analyses - chromosome counting 391
5.2 Ploidy determination using flow cytometry 397
6 Major media composition and basic equipment for DH laboratory
6.1 Major media composition 405
6.2 Basic equipment for maize microspore culture laboratory 415
List of contributors 417
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