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Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods from academia with a potential for scale-up. Also treated are various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology.
The book contains black-and-white illustrations.
This book reviews both the conventional and novel isolation techniques in downstream processing of protein molecules as used in industrial biotechnology. It also provides an introduction of novel techniques used in academia at the laboratory level that has potential for scale-up. Finally, the book brings together various strands of a biological process downstream which are essential for a successful application of a product license including the validation of DSP stages as well as the design and validation of viral clearance stages in a purification process.
"This valuable book is the result of collective work of mostly corporate authors with important experience in downstream processing of proteins in industrial and biotechnological environments....It may be good textbook in industrial biotechnology courses especially because of ample prools given in this book....The simplicity and clarity of the chapters is contains could be also of a great advantage for food science, which would certainly need to have good bench book....I would recommend highly this unpretentious but highly valuable book also to the readers active in our field."-Nahrung/Food
"a worthwhile addition to the reference collections of special and academic libraries that serve scientists interested in the study and isolation of medical products from natural materials."-E-streams Electronic Book Reviews
Downstream Processing in the Biotechnology Industry: An Overview, Manohar Kalyanpur. Microbial Cell Disruption by High-Pressure Homogenization, Anton P. J. Middelberg. Protein Concentration and Buffer Exchange Using Ultrafiltration, Andrew L. Zydney and Ralf Kuriyel. High-Performance Tangential Flow Filtration for Protein Separations, Andrew L. Zydney and Ralf Kuriyel. Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation, Anton P. J. Middelberg. Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules, Mohamed A. Desai and Sandra P. Merino. Application of Chromatography in the Downstream Processing of Biomolecules, Mohamed A. Desai, Mark Rayner, Mike Burns, and Dervilla Bermingham. Affinity Ligand Technology: Optimal Protein Separations for Downstream Processing, Ken Jones and Dev Baines. Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules, Mohamed A. Desai. Expanded Bed Adsorption in the Purification of Biomolecules, Allan Lihme, Marie Hansen, Morten Olander, and Elias Zafirakos. Application of HPLC in the Purification of Biomolecules, Paul Bradley and Mohamed A. Desai. Countercurrent Chromatography of Proteins with Polymer Phase Systems, Yoichiro Shibusawa, Kazuhiro Matsuda, and Yoichiro Ito. Practical Aspects and Applications of Radial Flow Chromatography, Denise M. Wallworth. Sterile Filtration and Virus Filtration, Ralf Kuriyel and Andrew L. Zydney. Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products, Allan J. Darling. Validation of Sterilizing Filters in the Biotechnology Industry, Manohar Kalyanpur. Index.