Drosophila: Methods and Protocols / Edition 1

Drosophila: Methods and Protocols / Edition 1

by Christian Dahmann
     
 

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ISBN-10: 1588298175

ISBN-13: 9781588298171

Pub. Date: 07/28/2008

Publisher: Springer-Verlag New York, LLC

With its long-standing tradition as a model organism, many techniques using Drosophila melanogaster have been established and continue to be developed, while the recent invention of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous recombination demonstrates the ongoing efforts to broaden the technical repertoire. In

Overview

With its long-standing tradition as a model organism, many techniques using Drosophila melanogaster have been established and continue to be developed, while the recent invention of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous recombination demonstrates the ongoing efforts to broaden the technical repertoire. In Drosophila: Methods and Protocols, leading experts contribute a collection of protocols covering standard techniques as well as novel methods. As a volume in the highly successful Methods in Molecular Biology series, the chapters contain brief introductions to the subject matter, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and essential, Drosophila: Methods and Protocols allows novices and experts alike to tap into the power of one hundred years of expertise involving this vital model system.

Product Details

ISBN-13:
9781588298171
Publisher:
Springer-Verlag New York, LLC
Publication date:
07/28/2008
Series:
Methods in Molecular Biology Series, #420
Edition description:
2008
Pages:
438
Product dimensions:
6.20(w) x 9.00(h) x 0.90(d)

Table of Contents

Methods in Molecular Biology Series Editor: John M. Walker Volume: Drosophila: Methods and Protocols Edited by Christian Dahmann, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany Contents Preface Contributors 1 Drosophila melanogaster and the Development of Biology in the XX century Alfonso Martinez Arias 2 Getting started: An overview on raising and using Drosophila Hugo Stocker and Peter Gallant 3 FlyBase: A database for the Drosophila research community Rachel Drysdale 4 The use of P-element transposons to generate transgenic flies André Bachmann and Elisabeth Knust 5 The GAL4 system: a versatile system for the expression of genes David A. Elliott and Andrea H. Brand 6 P-element mutagenesis Thomas Hummel and Christian Klämbt 7 EMS Screens: From mutagenesis to screening and mapping Christian Bökel 8 RNAi screening in cultured Drosophila cells Sandra Steinbrink and Michael Boutros 9 Methods for homologous recombination in Drosophila Keith A. Maggert, Wei J Gong, and Kent G. Golic 10 Recombinases and their use in gene activation, gene inactivation, and transgenesis Johannes Bischof and Konrad Basler 11 Cuticle preparation of Drosophila embryos and larvae Cyrille Alexandre 12 Immunolabelling of embryos H.- Arno J. Müller 13 Imaging cellular and molecular dynamics in live embryos using fluorescent proteins Matthieu Cavey and Thomas Lecuit 14 Analysis of neuromuscular junctions: histology and in vivo imaging Andreas Schmid and Stephan J. Sigrist 15 Immunolabelling of imaginal discs Thomas Klein 16 Imaging Drosophila pupal wing morphogenesis Anne-Kathrin Classen, Benoit Aigouy, Angela Giangrande, and SuzanneEaton 17 Microscopic analysis of the adult Drosophila retina using semi thin plastic sections Konstantin Gaengel and Marek Mlodzik 18 Fluorescent in situ hybridization protocols in Drosophila embryos and tissues Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause 19 Linear RNA amplification for the production of microarray hybridization probes Ansgar Klebes and Thomas B. Kornberg 20 An overview of the identification, detection, and functional analysis of Drosophila microRNAs Nicholas Sokol 21 Extraction and immunoblotting of proteins from embryos Andreas Wodarz 22 Purification of Drosophila protein complexes for mass spectrometry Christoph Jüschke and Jürgen A. Knoblich 23 Mass production of Drosophila embryos and chromatographic purification of native protein complexes Natascha Kunert and Alexander Brehm 24 Flow cytometric analysis of Drosophila cells Aida Flor A. de la Cruz and Bruce A. Edgar 25 Drosophila cell lines as model systems and as an experimental tool Buzz Baum and Lucy Cherbas Index

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