Drosophila: Methods and Protocols / Edition 1

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With its long-standing tradition as a model organism, many techniques using Drosophila melanogaster have been established and continue to be developed, while the recent invention of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous recombination demonstrates the ongoing efforts to broaden the technical repertoire. In Drosophila: Methods and Protocols, leading experts contribute a collection of protocols covering standard techniques as well as novel methods. As a volume in the highly successful Methods in Molecular BiologyT series, the chapters contain brief introductions to the subject matter, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and essential, Drosophila: Methods and Protocols allows novices and experts alike to tap into the power of one hundred years of expertise involving this vital model system. Written for: Geneticists, developmental biologists, molecular and cell biologists, graduate students and post docs

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Product Details

  • ISBN-13: 9781588298171
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 7/28/2008
  • Series: Methods in Molecular Biology Series, #420
  • Edition description: 2008
  • Edition number: 1
  • Pages: 438
  • Product dimensions: 6.20 (w) x 9.00 (h) x 0.90 (d)

Table of Contents

Preface     v
Contributors     ix
Drosophila melanogaster and the Development of Biology in the 20th Century   Alfonso Martinez Arias     1
Getting Started: An Overview on Raising and Handling Drosophila   Hugo Stocker   Peter Gallant     27
FlyBase: A Database for the Drosophila Research Community   Rachel Drysdale$dthe FlyBase Consortium     45
The Use of P-Element Transposons to Generate Transgenic Flies   Andre Bachmann   Elisabeth Knust     61
The GAL4 System: A Versatile System for the Expression of Genes   David A. Elliott   Andrea H. Brand     79
P-Element Mutagenesis   Thomas Hummel   Christian Klambt     97
EMS Screens: From Mutagenesis to Screening and Mapping   Christian Bokel     119
RNAi Screening in Cultured Drosophila Cells   Sandra Steinbrink   Michael Boutros     139
Methods for Homologous Recombination in Drosophila   Keith A. Maggert   Wei J. Gong   Kent G. Golic     155
Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis   Johannes Bischof   Konrad Basler     175
Cuticle Preparation of Drosophila Embryos and Larvae   CyrilleAlexandre     197
Immunolabeling of Embryos   H.-Arno J. Muller     207
Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins   Matthieu Cavey   Thomas Lecuit     219
Analysis of Neuromuscular Junctions: Histology and In Vivo Imaging   Andreas Schmid   Stephan J. Sigrist     239
Immunolabeling of Imaginal Discs   Thomas Klein     253
Imaging Drosophila Pupal Wing Morphogenesis   Anne-Kathrin Classen   Benoit Aigouy   Angela Giangrande   Suzanne Eaton     265
Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections   Konstantin Gaengel   Marek Mlodzik     277
Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues   Eric Lecuyer   Neela Parthasarathy   Henry M. Krause     289
Linear RNA Amplification for the Production of Microarray Hybridization Probes   Ansgar Klebes   Thomas B. Kornberg     303
An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs   Nicholas S. Sokol     319
Extraction and Immunoblotting of Proteins From Embryos   Andreas Wodarz     335
Purification of Drosophila Protein Complexes for Mass Spectrometry   Christoph Juschke   Jurgen A. Knoblich     347
Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes   Natascha Kunert   Alexander Brehm     359
Flow Cytometric Analysis of Drosophila Cells   Aida Flor A. de la Cruz   Bruce A. Edgar     373
Drosophila Cell Lines as Model Systems and as an Experimental Tool   Buzz Baum   Lucy Cherbas     391
Phenotypic Markers in Drosophila   Jennifer Childress   Georg Halder     425
Index     429
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