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From The CriticsReviewer: Omer Iqbal, MD, FACC, FESC(Loyola University Medical Center)
Description: This book provides authoritative and current information on the binding of new generations of DNA sequence reading compounds including triplex forming oligonucleotides and minor groove binding ligands to specific DNA sequences as anti-gene agents. This is an update of the previous edition published 12 years ago.
Purpose: This book is expected to be useful for established workers who wish to broaden their experimental repertoire, as well as for those who are new to the field and need expert advice and guidance.
Audience: The intended audience includes postgraduates, postdoctoral workers, and established scientists working in the field of drug-DNA interactions. However, even clinical investigators such as oncologists may find this book quite useful. Chapters are written by leading experts.
Features: Conveniently divided into 19 chapters, the book covers a wide range of approaches, from the cellular to the structural. The first nine chapters deal with various biophysical techniques such as thermal melting studies, capillary electrophoresis, and circular and linear dichroism of drug-DNA interactions, and other important techniques for quantifying drug-DNA interactions described in molecular and atomic detail. Other chapters cover molecular and cellular approaches of assessing the strength and mode of binding, the sequence selectivity, and their effect on biological systems. The final chapter details a series of biochemical, biophysical and cellular assays used to evaluate the activity of new compounds and to assess their suitability for examination in xenograft models of human cancer.
Assessment: This well-illustrated book of chapters written by leading experts will be extremely useful for postgraduates, post-doctoral fellows, established scientists, and some clinicians interested in the field of drug-DNA interactions. Given the significant advances in this field since the publication of the first edition in 1998, this update will be welcomed by students and justifies replacement.