Ever so slightly, the quality of PRC Pictures' film output improved as the 1940s rolled on. In PRC's Dixie Jamboree, Frances Langford plays Susan Jackson, the daughter of a showboat skipper (Guy Kibbee). Captain Jackson's vessel, the Ellabelle, is the last of the Mississippi showboats, and as such has become a refuge for such social outcasts as con artists Tony (Lyle Talbot) and Curly (Frank Jenks), itinerant musician Jeff Calhoun (Eddie Quillan), and ham actors Yvette (Fifi D'Orsay) and the Professor (Charles Butterworth). When Jackson inadvertently picks up a shipment of whiskey, Tony and Curly, assuming that the captain is a wealthy distiller, plan to hijack the boat and its cargo. All of this is set to the music of Cajun ditties, black spirituals, and lively cakewalks, performed con brio by Frances Langford and company.