The ELISA Guidebook: Second Edition / Edition 2

The ELISA Guidebook: Second Edition / Edition 2

by John R. Crowther
     
 

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ISBN-10: 1603272534

ISBN-13: 9781603272537

Pub. Date: 12/12/2008

Publisher: Springer-Verlag New York, LLC

The Enzyme Linked Immunosorbent Assay (ELISA) continues, as predicted, to be the predominant assay used in biomedical and agricultural research. While little has changed in the principles of the ELISAs used since the publication of The ELISA Guidebook, many technological advantages, including the increasing number of reagents available for exploitation and the

Overview

The Enzyme Linked Immunosorbent Assay (ELISA) continues, as predicted, to be the predominant assay used in biomedical and agricultural research. While little has changed in the principles of the ELISAs used since the publication of The ELISA Guidebook, many technological advantages, including the increasing number of reagents available for exploitation and the larger spectrum of instruments to measure the coloured products of ELISAs, have greatly benefited the field. Other testing technologies using molecular techniques, predominantly the conventional and more and more often, real time PCR, are rapidly evolving to help diagnose and differentiate disease agents and identify substances present in exquisitely small amounts. The ELISA is still needed either alone in mass screening, such as serological assays, or used in tandem with molecular techniques to fully evaluate the molecular and antigenic nature of analytes.

John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. This Edition enlarges on charting methods for assessing the indirect ELISA, ruggedness and robustness of tests, aspects of kit use and validation, and internal quality control and external quality management of data. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Wherever possible, helpful written explanations are provided along with copious diagrams.

With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook, Second Edition offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply ELISA methodology successfully in day-to-day basic and clinical research.

Product Details

ISBN-13:
9781603272537
Publisher:
Springer-Verlag New York, LLC
Publication date:
12/12/2008
Series:
Methods in Molecular Biology Series, #516
Edition description:
2nd ed. 2009
Pages:
566
Product dimensions:
7.70(w) x 10.30(h) x 1.20(d)

Table of Contents

1) Overview of ELISA in Relation to Other Disciplines

2) Systems in ELISA

3) Stages in ELISA

4) Titration of Reagents

5) Theoretical Considerations

6) Practical Exercises

7) Monoclonal Antibodies

8) Validation of Diagnostic Tests for Infectious Diseases

9) Charting Methods for Internal Quality Control for Competition ELISA

10) Charting Methods for Internal Quality Control of Indirect ELISA

11) Ruggedness and Robustness of Tests: Aspects of Kit Use and Validation

12) More Advanced Statistical Methods for Quality Assurance, Test Validation and Interpretation

13) Internal Quality Control and External Quality Management of Data in Practice

14) Immunochemical Techniques

15) Test Questions

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