ELISA: Theory and Practice / Edition 1

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Overview

ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation.

ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.

The book contains black-and-white illustrations.

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Editorial Reviews

Doody's Review Service
Reviewer: Venk Lakshminarayanan(Rush Medical College of Rush University)
Description: This book is designed to discuss the applications of the enzyme-linked immunosorbent assay (ELISA). The book is divided into two portions, one dealing with the basic principles and theory of ELISA and the other addressing the practical applications.
Purpose: The purpose is to introduce the experimenter to ELISA. It is directed toward individuals with varying degrees of work with this assay system. The book can serve as both a teaching tool and reference manual for individuals involved with the ELISA. The overall objective of the book is met and provides useful and necessary insight.
Audience: The book is targeted toward any individual who uses the ELISA system of assay. As stated previously, it will serve as a good teaching tool as well as a reference manual for more in-depth use of the system.
Features: The book contains many figures, including graphs, tables of data, and schematic drawings depicting the interaction of antibodies and antigens. The only drawback to the illustrations is the shading. These figures are done in black-and-white, and the shading makes some of the figures confusing.
Assessment: I find the book useful and insightful. It provides helpful information for all individuals, from the novice to the experienced. Its best feature is the organization of material. By separating the book into two primary sections, the user can focus on the theoretical or practical applications of the ELISA. I consider it a worthwhile resource.
Venk Lakshminarayanan
This book is designed to discuss the applications of the enzyme-linked immunosorbent assay (ELISA). The book is divided into two portions, one dealing with the basic principles and theory of ELISA and the other addressing the practical applications. The purpose is to introduce the experimenter to ELISA. It is directed toward individuals with varying degrees of work with this assay system. The book can serve as both a teaching tool and reference manual for individuals involved with the ELISA. The overall objective of the book is met and provides useful and necessary insight. The book is targeted toward any individual who uses the ELISA system of assay. As stated previously, it will serve as a good teaching tool as well as a reference manual for more in-depth use of the system. The book contains many figures, including graphs, tables of data, and schematic drawings depicting the interaction of antibodies and antigens. The only drawback to the illustrations is the shading. These figures are done in black-and-white, and the shading makes some of the figures confusing. I find the book useful and insightful. It provides helpful information for all individuals, from the novice to the experienced. Its best feature is the organization of material. By separating the book into two primary sections, the user can focus on the theoretical or practical applications of the ELISA. I consider it a worthwhile resource.
Booknews
Introduces to scientists at all levels of expertise the principles of the most commonly used assay technique, known as Enzyme Linked Immunoabsorbent Assay. Provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in current science, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. Plastic comb- binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

3 Stars from Doody
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Product Details

  • ISBN-13: 9780896032798
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 2/22/2010
  • Series: Methods in Molecular Biology Series , #42
  • Edition description: 1995
  • Edition number: 1
  • Pages: 256
  • Product dimensions: 9.21 (w) x 6.14 (h) x 0.51 (d)

Table of Contents

Basic Immunology:

1. The Immune Response
2. Antigens
3. Adaptive Immunity and Clonal Selection
4. Antibodies
5. Antigenic Commonness

Basic Principles of ELISA:

1. Reaction Schemes
2. Direct ELISA
3. Indirect ELISA
4. Sandwich ELISA
5. Competition ELISA
6. Choice of Assays

Stages in ELISA:

1. Solid-Phase
2. Washing
3. Addition of Reagents
4. Incubation
5. Blocking Conditions and Nonspecific Reactions
6. Enzyme Conjugates
7. Availability of Conjugates
8. Conjugation with Enzymes
9. Development of Label
10. Stopping Reactions
11. Reading
12. Practical Problems

Theoretical Considerations:

1. Setting Up and Use of ELISA
2. What Is Already Known
3. Complexity of Problems
4. Antigenic Considerations
5. Other Techniques
6. Units
7. Making Dilutions
8. Pipets
9. Molarities

Direct ELISA:

Titration of Antigen and Antibody:

1. Learning Principles
2. Reaction Scheme
3. Basis of Assay
4. Materials and Equipment
5. Practical Details
6. Data Explained
7. Aspects of Assay Described
8. Conclusions

Indirect ELISA:

1. Learning Principles
2. Basis of Assay
3. Materials and Reagents
4. Practical
5. Use of Indirect ELISA to Titrate Antibodies
6. Use of Indirect ELISA to Determine the Positivity of Sera at Single Dilution

Use of Antibodies on Solid-Phase in Capture ELISA:

1. Use of Capture ELISA to Detect and Titrate Antigen
2. Reaction Scheme
3. Basis of Assay
4. Materials
5. Methods
6. Titration of Capture Antibody Using IgG
7. Titration of Capture Antibody when Used as Whole Serum
8. Use of Capture ELISA to Detect Antigens
9. Use of Capture ELISA to Detect and Titrate Antibodies

Competitive ELISA:

1. General Information
2. Direct Competitive ELISA for Antigen Detection and Quantification
3. Indirect Assay-Antigen Competition
4. Indirect Competition Assay for Antibody Detection
5. Indirect Assay Competition for Antibody-Detection Using a Single Dilution of Test Serum
6. Overall Conclusions on Competition Assays

Immunochemical Techniques:

1. Introduction
2. Labeling Antibodies with Enzymes
3. Coupling Antibodies to Alkaline Phosphatase
4. Preparation of Immunoglobulins
5. Immunosorbents
6. Production of Antisera

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