Embryonic Stem Cells: A Practical Approach

Embryonic Stem Cells: A Practical Approach

by Elena Notarianni, Martin J. Evans
     
 

ISBN-10: 0198550006

ISBN-13: 9780198550006

Pub. Date: 08/28/2006

Publisher: Oxford University Press, USA

The groundbreaking isolation of embryonic stem cells (or 'ES cells') of the mouse in the early 1980s triggered a sustained expansion of global research into their exploitation. This led to the routine genetic engineering of the mouse and revolutionised our understanding of biological processes in the context of the whole animal. ES cell biology remains a crucial

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Overview

The groundbreaking isolation of embryonic stem cells (or 'ES cells') of the mouse in the early 1980s triggered a sustained expansion of global research into their exploitation. This led to the routine genetic engineering of the mouse and revolutionised our understanding of biological processes in the context of the whole animal. ES cell biology remains a crucial and growing area of research with far-reaching implications for developmental and comparative biology as well as for human health.
This book serves as a primer to ES cells, their derivation and experimental manipulation. It contains a broad compendium of methods of direct relevance to both graduate students and specialist researchers. An introductory chapter by the principle originator of ES cell research outlines the fundamentals and charts the development of the field. This is followed by comprehensive coverage of state-of-the art techniques for ES cell manipulation, with the mouse as the experimental paradigm, and by recent innovations with ES cells from human and non-human primates. ES cell-based therapies for otherwise intractable diseases are now being developed with the present challenge to control ES cell growth and differentiation for applications such as cell transplantation - a recurrent theme in this book. As a volume in the Practical Approach Series, the emphasis is on current methods from recognized experts.

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Product Details

ISBN-13:
9780198550006
Publisher:
Oxford University Press, USA
Publication date:
08/28/2006
Series:
Practical Approach Series, #270
Pages:
360
Product dimensions:
9.80(w) x 7.50(h) x 1.00(d)

Table of Contents

1Introduction2
2Procedures for deriving ES cell lines from the mouse7
3Production of ES cell-derived mice41
4Generating animal models of human mitochondrial genetic disease using mouse ES cells72
5Controlling the differentiation of mouse ES cells in vitro112
6In vitro differentiation of mouse ES cells into muscle cells130
7In vitro differentiation of mouse ES cells into haematopoietic cells169
8Lineage selection and transplantation of mouse ES cell-derived neural precursors189
9In vitro differentiation of mouse ES cells into pancreatic and hepatic cells218
10Isolation and characterization of human ES cells238
11Differentiation of human ES cells260
12ES cell lines from the cynomolgus monkey (Macaca fascicularis)294

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