Description: This volume honors K.E. van Holde for his many contributions to studies of energetics of protein folding, protein assembly and biologic recognition, and ligand-ligand interaction. It includes 24 chapters by experts who cover areas including determination of the energetics of protein-bilayer interactions; molecular design of peptide ligands; study of the Bohr effect in hemoglobin interactions; and photoacoustic calorimetry of proteins and studies of interactions and folding of repressor proteins.
Purpose: This volume is meant to cover topics to which van Holde made contributions and to include newer methologic approaches and applications that should be of general interest. While some of these chapters are very specific, they illustrate the potential of these applications to other study systems. Nonetheless, this volume was not meant to be a general volume that focuses on only the most commonly used methods.
Audience: The audience could include anyone from student to experienced structural biologist or protein chemist. Each chapter is well written, very concise, yet very informative, and should be interesting reading for investigators at different levels of training. This book should be informative to anyone with a need to understand how to characterize protein structure and activities and an interest in the specific methods and applications presented.
Features: The features include many informative illustrations with very useful, very extensive bibliographies. The book is in the form of the attractive Methods in Enzymology series.
Assessment: This would be a worthwhile addition to the library of anyone with interests in energetics of protein folding and protein ligand interaction. Since the editors and contributors are internationally recognized experts, the content is very meaningful.