Environmental Microbiology: Methods and Protocols / Edition 1

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Overview

A collection of readily reproducible techniques for determining most of the important factors governing microorganisms and their habitats. Presented in step-by-step detail, these cutting-edge methods range from those for the study of marine organisms, to those for investigating microorganisms occurring in groundwater, to the biodiversity of yeasts found in remote environments. The novel prools for studying fermented milks are significant for investigators concerned with milk as an item of food for infants, small children, and even adults. Additional methods for the recovery and determination of nucleic acids and other compounds affecting, and affected by, microorganisms are given for certain enzymes produced by plant pathogens and for obtaining microbial species tolerant of such inhibitors as heavy metals.

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Product Details

  • ISBN-13: 9781617373701
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/9/2010
  • Series: Methods in Biotechnology Series , #16
  • Edition description: Softcover reprint of hardcover 1st ed. 2004
  • Edition number: 1
  • Pages: 426
  • Product dimensions: 9.00 (w) x 6.00 (h) x 0.98 (d)

Table of Contents

Part I. Communities and Biofilms

Isolation and Molecular Characterization of Seawater Bacteria

Juliana M. Benito, Gustavo A. Lovrich, Faustino Siñeriz, and Carlos M. Abate

Reverse Sample Genome Probing to Monitor Microbial Communities

E. Anne Greene and Gerrit Voordouw

T-RFLP Analysis: A Rapid Fingerprinting Method for Studying Diversity, Structure, and Dynamics of Microbial Communities

Werner Liesack and Peter F. Dunfield

Assessing Bacterial DNA Content in Aquatic Systems

Garrett W. Perney, Betsy R. Robertson, and D. K. Button

Multiplexed Identification and Quantification of Analyte DNAs in Environmental Samples Using Microspheres and Flow Cytometry

Mary Lowe, Alex Spiro, Anne O. Summers, and Joy Wireman

Molecular Characterization of Microbial Communities From Marine Environments

Nicolás G. Barengo, Juliana M. Benito, and Carlos M. Abate

Yeasts: Ecology in Northwest Argentina

Lucía I. C. de Figueroa, M. Alejandra Martínez, and John F. T. Spencer

Aeromonads in Environmental Waters

Anavella Gaitan Herrera

Development of a Vital Fluorescent Staining Method for Monitoring Bacterial Transport in Subsurface Ground Water

Mark E. Fuller

Bench Scale Flow Cell for Nondestructive Imaging of Biofilms

Eric S. Gilbert and Jay D. Keasling

Part II. Fermented Milks

a-Galactosidase Assay in Fermented Soymilk Products

Marisa S. Garro, Graciela Font de Valdez, and Graciela Savoy de Giori

Considerations to Avoid the Overestimation of Exopolysaccharides Produced by Lactic Acid Bacteria

María Inés Torino and Graciela Font de Valdez

Determination of Oligosaccharides in Fermented Soymilk Products by High-Performance Liquid Chromatography

Marisa S. Garro, Graciela Font de Valdez, and Graciela Savoy de Giori

Evaluation of Minimal Nutritional Requirements of Lactic Acid Bacteria Used in Functional Foods

Elvira M. Hébert, Raúl R. Raya, and Graciela Savoy de Giori

Part III. Nucleic Acids, Recovery, and Determination

A Simple Method for Obtaining DNA Suitable for RAPD Analysis From Azospirillum

Raúl O. Pedraza and Juan C. Díaz Ricci

PCR Fingerprinting of rRNA Intergenic Spacer Regions for Molecular Characterization of Environmental Bacteria Isolates

M. Alejandra Martínez and Faustino Siñeriz

A DNA-DNA Hybridization Method for the Detection and Quantification of Specific Bacterial Taxa in Natural Environments

Christopher E. Bagwell and Charles R. Lovell

13C and 1H NMR Study of the Glycogen Futile Cycle in Fibrobacter succinogenes

Anne-Marie Delort, Genevieve Gaudet, and Evelyne Forano

Chemical Analysis and Biological Removal of Wood Lipids Forming Pitch Deposits in Paper Pulp Manufacturing

Ana Gutiérrez, José C. del Río, and Ángel T. Martínez

Propolis: Chemical Micro-Heterogeneity and Bioactivity

José Domingos Fontana, Juliana Adelmann, Mauricio Passos, Marcelo Maraschin, Cristina A. de Lacerda, and Fernando Mauro Lanças

Enzymatic Saccharification of Cellulosic Materials

Luiz Pereira Ramos and José Domingos Fontana

Molecular Identification of Microbial Populations in Petroleum-Contaminated Groundwater

Kazuya Watanabe, Natsuko Hamamura, and Nobuo Kaku

Identification of Copper-Resistant Microorganisms by PCR

Virginia H. Albarracín, Juliana M. Benito, Manuel Siñeriz Louis, María J. Amoroso, and Carlos M. Abate

Selection of Tolerant Heavy Metal Yeasts From Different Polluted Sites

Liliana Villegas, María J. Amoroso, and Lucía I. C. de Figueroa

Medium for Differential Enumeration of Lactobacillus casei and Lactobacillus acidophilus From Lyophilized Mixed Cultures

Mónica M. Locascio, Rosalía Alesso, Vilma I. Morata, and Silvia N. González

Silk as a Means of Recovering Bacteriocin From a Culture Medium

M. Carina Audisio and María C. Apella

Optimization of a Method for Isolating Plasmid DNA From Lactic Acid Bacteria From Wine

Fabiana M. Saguir and María C. Manca de Nadra

Simultaneous and Sequential Methods to Study Interactions Between Yeast and Lactic Acid Bacteria From Wine

Marta E. Farías and María C. Manca de Nadra

Method for Determining Lindane Concentration in Water and Solid Samples

Claudia S. Benimeli, Adriana P. Chaile, and María J. Amoroso

Extracellular Hydrolytic Enzymes Produced by Yeasts

Fabio Vazquez, Martha Dina Vallejo Herrera, Lucía I. C. de Figueroa, and María Eugenia Toro

Extracellular Hydrolytic Enzymes Produced by Phytopathogenic Fungi

Martha Dina Vallejo Herrera, María Eugenia Toro, Lucía I. C. de Figueroa, and Fabio Vazquez

An Analysis of Microorganisms in Environments Using Denaturing Gradient Gel Electrophoresis

Paul W. Baker and Shigeaki Harayama

Testing for Evolutionary Correlations in Microbiology Using Phylogenetic Generalized Least Squares

Scott R. Miller

Conjugated Linoleic Acid Detection Produced by Dairy Starter Cultures

Carina Van Nieuwenhove, Silvia N. González, and A|icia Bardón

Part IV. Reviews

Techniques for Manipulating the Bacterial Endophyte Bacillus mojavensis

Charles W. Bacon and Dorothy M. Hinton

Engineering of Bacteria Using the Vitreoscilla Hemoglobin Gene to Enhance Bioremediation of Aromatic Compounds

Benjamin C. Stark, Dale A. Webster, and Krishna R. Pagilla

The Use of Chemical Shift Regents and 23Na NMR to Study Sodium Gradients in Microorganisms

Anne-Marie Delort, Genevieve Gaudet, and Evelyne Forano

Denaturing Gradient Gel Electrophoresis (DGGE) as a Fingerprinting Tool for Analyzing Microbial Communities in Contaminated Environments

Laurent Eyers, Spiros N. Agathos, and Said El Fantroussi

Index

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