Environmental Monitoring of Bacteria

Overview

Clive Edwards and his distinguished panel of researchers bring together a collection of detailed molecular methods for the environmental monitoring of bacterial populations. These state-of-the-art methods include techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations. Many of the methods enable bacteria to be monitored in their natural environments without culturing and specifically address the difficult problem that only ...

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Overview

Clive Edwards and his distinguished panel of researchers bring together a collection of detailed molecular methods for the environmental monitoring of bacterial populations. These state-of-the-art methods include techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations. Many of the methods enable bacteria to be monitored in their natural environments without culturing and specifically address the difficult problem that only a small proportion of bacteria in any ecosystem can be cultured by traditional microbiological methods. Environmental Monitoring of Bacteria takes full advantage of the exciting research in recent years that has challenged some long-held principles of traditional microbiology and gives timely and practical access to techniques that allow the study of bacterial ecology as communities, single cells, or at the molecular level.

The book contains black-and-white illustrations.

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Editorial Reviews

Charles E. Edmiston
This text is a discussion of the various techniques that are currently available for the identification and characterization of microbial populations in terrestrial and aquatic environments. The purpose is to provide scientists and students with a concise, state-of-the-art resource describing the strategies for the collection, identification, and characterization of microbial populations in natural environments. This book is directed to the environmental microbiologist, soil scientist, and graduate student engaged in studying natural terrestrial or aquatic microbial populations. This text covers several important areas including collection strategies for terrestrial and aquatic environments, magnetic particle separation techniques, molecular techniques such as DNA extraction and sequencing, fluorescent PCR/RFLP, recovery of ribosomal RNA, and analysis and confocal laser scanning microscopy of environmental samples. All of the techniques are well described with appropriate and numerous references for additional reading. This is a timely and well conceived reference text for both scientists and students who are interested in the analysis of natural (environmental) microbial populations on either the community or molecular level. The editor has assembled an exceptional group of contributors whose combined efforts have made this text a truly outstanding contribution to the field of environmental microbial monitoring.
Booknews
Twenty contributions cover molecular methods for the environmental monitoring of bacterial populations and offer techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations. Many of the methods discussed enable the monitoring of bacteria in their natural environments without culturing and address the problem that only a small proportion of bacteria in any ecosystem can be cultured by traditional microbiological methods. Intended for microbiologists, biologists, and clinical bacteriologists. Annotation c. by Book News, Inc., Portland, Or.
Doody's Review Service
Reviewer: Charles E. Edmiston, Jr., PhD (Medical College of Wisconsin)
Description: This text is a discussion of the various techniques that are currently available for the identification and characterization of microbial populations in terrestrial and aquatic environments.
Purpose: The purpose is to provide scientists and students with a concise, state-of-the-art resource describing the strategies for the collection, identification, and characterization of microbial populations in natural environments.
Audience: This book is directed to the environmental microbiologist, soil scientist, and graduate student engaged in studying natural terrestrial or aquatic microbial populations.
Features: This text covers several important areas including collection strategies for terrestrial and aquatic environments, magnetic particle separation techniques, molecular techniques such as DNA extraction and sequencing, fluorescent PCR/RFLP, recovery of ribosomal RNA, and analysis and confocal laser scanning microscopy of environmental samples. All of the techniques are well described with appropriate and numerous references for additional reading.
Assessment: This is a timely and well conceived reference text for both scientists and students who are interested in the analysis of natural (environmental) microbial populations on either the community or molecular level. The editor has assembled an exceptional group of contributors whose combined efforts have made this text a truly outstanding contribution to the field of environmental microbial monitoring.

3 Stars from Doody
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Product Details

  • ISBN-13: 9781617370779
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/19/2010
  • Series: Methods in Biotechnology Series, #12
  • Edition description: Softcover reprint of hardcover 1st ed. 1999
  • Edition number: 1
  • Pages: 333
  • Product dimensions: 0.77 (w) x 6.00 (h) x 9.00 (d)

Table of Contents

Some Problems Posed by Natural Environments for Monitoring Microorganisms, Clive Edwards. Sampling Sediment and Soil: Use of Coring Devices, Roger Pickup, Glenn Rhodes, and Grahame Hall. Sampling Water Bodies: Tangetial Flow Filtration, Roger Pickup, Helen Mallinson, and Glenn Rhodes. Dielectrophoresis, D. W. Pimbley, P. D. Patel, and C. J. Robertson. Flow Cytometry and Cell Sorting: Rapid Analysis and Separation of Individual Bacterial Cells from Natural Environments, Jonathan Porter. Magnetic Particle-Based Separation Techniques for Monitoring Bacteria from Natural Environments, Jonathan Porter and Roger Pickup. DNA Extraction from Natural Environments, Kenneth D. Bruce, Peter Strike, and Donald A. Ritchie. Automated Sequencing of DNA Retrieved from Environmental Samples, Mathew Upton. Analysis of DNA Sequences, Mathew Upton. Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism Monitoring of Genes Amplified Directly from Bacterial Communities in Soils and Sediments, Kenneth D. Bruce and Mark R. Hughes. Recovery and Analysis of Ribosomal RNA Sequences from the Environment, Ian M. Head. Application of Denaturing Gradient Gel Electrophoresis to Microbial Ecology, Richard Hastings. Reporter Gene Expression for Monitoring Microorganisms in the Environment, James R. Firth. Characterizing Microorganisms in the Environment by Fatty Acid Analysis, Ian P. Thompson, Mark J. Bailey, and Andrew K. Lilley. Fluorescent In Situ Hybridization and the Analysis of the Single Cell, Anthony G. O'Donnell and Andrew S. Whiteley. Specific Detection, Viability Assessment, and Macromolecular Staining of Bacteria for Flow Cytometry, Jonathan Porter. Confocal Laser Scanning Microscopy of Environmental Samples, David Lloyd, Anthony J. Hayes, and James R. Ralphs. Monitoring Microbial Activities Using Membrane Inlet Mass Spectrometry, James R. Firth and Clive Edwards. Experimental Biofilms and Their Applications in the Study of Environmental Processes, Joanna C. Rayner and Hilary M. Lappin-Scott. Establishment of Experimental Biofilms Using the Modified Robbins Device and Flow Cells, Luanne Hall-Stoodley, Joanna C. Rayner, Paul Stoodley, and Hilary M. Lappin-Scott. Index.

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