Enzyme Assays / Edition 2

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Overview

Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis.

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Editorial Reviews

From The Critics
Eisenthal and Danson (both of the Department of Biology and Biochemistry, U. of Bath, UK) explain commonly used and newer, experimental techniques of measuring the rate of enzyme-catalyst reaction as a way to begin to define the functional roles of genetic proteins. After an introductory chapter dealing with fundamental principles, chapters look at photometric, radiometric, high performance liquid chromatographic, and electrochemical techniques. Further topics include techniques for enzyme extraction, determination of active site concentration, high throughput screening, and statistical analysis of enzyme kinetic data. Annotation c. Book News, Inc., Portland, OR
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Product Details

  • ISBN-13: 9780199638208
  • Publisher: Oxford University Press, USA
  • Publication date: 6/28/2002
  • Series: Practical Approach Series , #257
  • Edition description: REV
  • Edition number: 2
  • Pages: 302
  • Product dimensions: 9.60 (w) x 7.40 (h) x 0.70 (d)

Table of Contents

List of protocols
Abbreviations
1 Principles of enzyme assay and kinetic studies 1
2 Photometric assays 49
3 Radiometric assays 79
4 High performance liquid chromatographic assays 103
5 Electrochemical assays: to oxygen electrode 141
6 Electrochemical assays: the nitric oxide electrode 149
7 Electrochemical assays: the pH-stat 157
8 Enzyme assay after gel electrophoresis 171
9 Techniques for enzyme extraction 209
10 Determination of active site concentration 225
11 High throughput screening - considerations for enzyme assays 235
12 Statistical analysis of enzyme kinetic data 249
List of suppliers 269
Enzyme index 273
General index 277
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