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Enzymes in Nonaqueous Solvents: Methods and Protocols / Edition 1

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In Enzymes in Nonaqueous Solvents: Methods and Prools, leading chemists, biochemists, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic transformations under nonaqueous conditions. Each method includes detailed step-by-step instructions for its successful completion, a list of materials, and ancillary notes that explain the scientific basis of the procedure, as well as troubleshooting. Also provided are a generic description of key reactions, advice on biocatalyst preparation, discussion of reaction conditions, and instructions on bioreactor design.

Comprehensive and state-of-the-art, Enzymes in Nonaqueous Solvents: Methods and Prools offers today's synthetic chemists, biochemists, and process engineers all the essential information needed to carry out enzymatic reactions in nonaqueous media, as well as to successfully scale up to production quantities.

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Editorial Reviews

Chemists, biochemists, biotechnologists, and process engineers summarize methods for executing enzymatic transformations under nonaqueous conditions. Each method includes detailed step-by-step instructions, a list of materials, notes explaining the scientific basis of the procedure, and troubleshooting tips. Also provided are a generic descriptions of key reactions, advice on biocatalyst preparation, discussion of reaction conditions, and instructions on bioreactor design. Chapters are in sections on control of enzyme activity in nonaqueous solvents, synthetic applications, and reaction systems and bioreactor design. Vulfson is affiliated with the Institute of Food Research. Annotation c. Book News, Inc., Portland, OR (
From the Publisher
"The handbook provides detailed descriptions of a variety of methods used for performing enzymatic reactions in nonaqueous media." - Journal of the American Chemical Society

"...includes detailed descriptions of the latest technologies used for biocatalysis in nearly anhydrous systems. Numerous specific enzymatic reactions, bioreactors, and useful techniques are carefully explained in a format intelligible to most readers in spite of the great diversity of the fields covered. This work of reference will be extremely valuable to those interested in enzymatic and asymmetric synthesis." - Natural Products Reports

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Product Details

  • ISBN-13: 9780896039292
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 3/16/2001
  • Series: Methods in Biotechnology Series , #15
  • Edition description: 2001
  • Edition number: 1
  • Pages: 679
  • Product dimensions: 9.21 (w) x 6.14 (h) x 1.50 (d)

Table of Contents

Part I

Control of Enzyme Activity in Nonaqueous Solvents
Peter J. Halling

Salt-Induced Activation of Enzymes in Organic Solvents: Optimizing the Lyophilization Time and Water Content
Michael T. Ru, Jonathan S. Dordick, Jeffrey A. Reimer, and Douglas S. Clark

Imprinting Enzymes for Use in Organic Media
Joseph O. Rich and Jonathan S. Dordick

Entrapment of Biocatalysts by Prepolymer Methods
Atsuo Tanaka and Takamitsu Iida

Microencapsulation of Enzymes and Cells for Nonaqueous Biotransformations
Jeffrey A. Khan and Evgeny N. Vulfson

Immobilization of Lipases on Hydrogels
Abu Bakar Salleh, Norhaizan M. Esa, Mahiran Basri, Che Nyonya A. Razak, Wan Md Zin W. Yunus, and Mansor Ahmad

Polyethylene Glycol-Modified Enzymes in Hydrophobic Media
Ayako Matsushima, Yoh Kodera, Misao Hiroto, Hiroyuki Nishimura, and Yuji Inada

Chemical Modification of Lipase for Use in Ester Synthesis
Mahiran Basri, Kamaruzaman Ampon, Che Nyonya A. Razak, and Abu Bakar Salleh

Preparation and Properties in Organic Solvents of Noncovalent PEG-Enzyme Complexes
Francesco Secundo, Gianluca Ottlina, and Giacomo Carrea

Preparations of a Lipid-Coated Enzyme and Activity for Reverse Hydrolysis Reactions in Homogeneous Organic Media
Toshiaki Mori and Yoshio Okahata

Very High Activity Biocatalysts for Low-Water Systems: Propanol-Rinsed Enzyme Preparations
Barry D. Moore, Johann Partridge, and Peter J. Halling

Methods for Measurement and Control of Water in Nonaqueous Biocatalysis
George Bell, Peter J. Halling, Lindsey May, Barry D. Moore, Donald A. Robb, Rein Ulijn, and Rao H. Valivety

Water Activity Control in Organic Media by Equilibration Through Membranes
Ernst Wehtje and Patrick Adlercreutz

Water Activity Control for Lipase-Catalyzed Reactions in Nonaqueous Media
Joon Shick Rhee, Seok Joon Kwon, and Jeong Jun Han

Immobilization of Enzymes and Control of Water Activity in Low-Water Media: Properties and Applications of Celite R-640 (Celite Rods)
Lucia Gardossi

Enzyme Activity and Enantioselectivity Measurements in Organic Media
Amélie Ducret, Michael Trani, and Robert Lortie

Calorimetric Methods in Evaluating Hydration and Solvation of Solid Proteins Immersed in Organic Solvents
Mikhail Borisover, Vladimir Sirotkin, Dmitriy Zakharychev, and Boris Solomonov

Detection of Structural Changes of Enzymes in Nonaqueous Media by Fluorescence and CD Spectroscopy
Hideo Kise

The Effects of Crown Ethers on the Activity of Enzymes in Organic Solvents
Dirk-Jan van Unen, Johan F. J. Engbersen, and David N. Reinhoudt

Control of Acid-Base Conditions in Low-Water Media
Johann Partridge, Neil Harper, Barry D. Moore, and Peter J. Halling

Enzymatic Acylation of _-Butylglucoside in Nonaqueous Media
Marie-Pierre Bousquet, René-Marc Willemot, Pierre Monsan, and Emmanuel Boures

Part II

Synthetic Applications
Herbert L. Holland

Choosing Hydrolases for Enantioselective Reactions Involving Alcohols Using Empirical Rules
Alexandra N. E. Weissfloch and Romas J. Kazlauskas

Candida antarctica Lipase B: A Tool for the Preparation of Optically Pure Alcohols
Didier Rotticci, Jenny Ottosson, Torbjörn Norin, and Karl Hult

Enantioselective Lipase-Catalyzed Transesterifications in Organic Solvents
Fritz Theil

Pseudomonas cepacia Lipase-Catalyzed Enantioselective Acylation of 2-Substituted-1-alkanols in Organic Solvents
Patrizia Ferraboschi and Enzo Santaniello

Preparation of 2-, 3-, and 4-Methylcarboxylic Acids and the Corresponding Alcohols of High Enantiopurity by Lipase-Catalyzed Esterification
Per Berglund and Erik Hedenström

Optimization of Enzymatic

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