Enzymology and Molecular Biology of Carbonyl Metabolism 5

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Editorial Reviews

The July 1994 workshop, like previous workshops, was a satellite of the International Society for Biomedical Research on Alcoholism meeting and, like previous workshops, presented new information about the three enzyme systems--aldehyde dehydrogenase, aldo-keto reductase, and aldo-keto reductase aldehyde dehydrogenase. Among the topics in these proceedings are site directed mutagenesis to probe for active site components of liver mitochondrial aldehyde dehydrogenase; structure and mechanism of aldehyde reductase; and the alcohol dehydrogenase system. Annotation c. Book News, Inc., Portland, OR booknews.com
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Product Details

  • ISBN-13: 9781461358084
  • Publisher: Springer US
  • Publication date: 12/31/2013
  • Series: Advances in Experimental Medicine and Biology Series , #372
  • Edition description: Softcover reprint of the original 1st ed. 1995
  • Pages: 442
  • Product dimensions: 6.69 (w) x 9.61 (h) x 0.93 (d)

Table of Contents

1 Site Directed Mutagenesis to Probe for Active Site Components of Liver Mitochondrial Aldehyde Dehydrogenase 1
2 Substrate Binding Pocket Structure of Human Aldehyde Dehydrogenases: A Substrate Specificity Approach 9
3 Human Class 1 Aldehyde Dehydrogenase: Expression and Site-Directed Mutagenesis 17
4 Nitrate Esters as Inhibitors and Substrates of Aldehyde Dehydrogenase 25
5 Use of a Chromophoric Reporter Group to Probe the Active Site of Cytosolic Aldehyde Dehydrogenase 35
6 Studies of the Esterase Activity of Cytosolic Aldehyde Dehydrogenase Using Sterically Hindered and Cyclic Substrates 45
7 The Reduction of Propionic Anhydride by Aldehyde Dehydrogenase-Nadh Mixtures at pH 7 53
8 Cloning and Characterisation of the cDNA for Sheep Liver Cytosolic Aldehyde Dehydrogenase 61
9 Crystallization of Sheep Liver Cytosolic Aldehyde Dehydrogenase in a Form Suitable for High Resolution X-Ray Structural Analysis 67
10 Progress toward the Tertiary Structure of (Class 3) Aldehyde Dehydrogenase 71
11 UDP-Glucose Dehydrogenase: Structural Characteristics 79
12 Kinetic Studies on Class 3 Aldehyde Dehydrogenase from Bovine Cornea 85
13 Covalent Modification of Class 2 and Class 3 Aldehyde Dehydrogenase by 4-Hydroxynonenal 93
14 Constitutive and Overexpressed Human Cytosolic Class-3 Aldehyde Dehydrogenases in Normal and Neoplastic Cells/Secretions 103
15 Metabolism of Cyclophosphamide by Aldehyde Dehydrogenases 115
16 Tissue-Specific Expression and Preliminary Functional Analysis of the 5' Flanking Regions of the Human Mitochondrial Aldehyde Dehydrogenase (ALDH2) Gene 123
17 Transgenesis of the Aldehyde Dehydrogenase-2 (ALDH2) Locus in a Mouse Model and in Cultured Human Cells 131
18 Class 3 Aldehyde Dehydrogenase: A Northern Perspective in the Land Down Under 137
19 Studies on the Induction of Rat Class 3 Aldehyde Dehydrogenase 143
20 Mouse Class 3 Aldehyde Dehydrogenases 151
21 Cloning and Characterization of Genes Encoding Four Additional Human Aldehyde Dehydrogenase Isozymes 159
22 New Human Aldehyde Dehydrogenases 169
23 Retinoic Acid Synthesizing Enzymes in the Embryonic and Adult Vertebrate 173
24 Retinoic Acid Synthesis in the Developing Spinal Cord 185
25 Structure and Mechanism of Aldehyde Reductase 193
26 Expression of Human and Rat Carbonyl Reductase in E. coli: Comparison of the Recombinant Enzymes 203
27 Molecular Cloning and Sequencing of Mouse Hepatic 11[beta]-Hydroxysteroid Dehydrogenase/Carbonyl Reductase: A Member of the Short Chain Dehydrogenase Superfamily 211
28 Molecular Modelling Calculations on the Binding of D- and L-Xylose to Wild-Type Aldose Reductase and Its H110Q and HII0A Mutants 223
29 Stopped-Flow Studies of Human Aldose Reductase Reveal which Enzyme Form Predominates during Steady-State Turnover in Either Reaction Direction 229
30 Lysine Residues in the Coenzyme-Binding Region of Mouse Lung Carbonyl Reductase 241
31 Substrate Specificity and Kinetic Mechanism of Tetrahymena 20[alpha]-Hydroxysteroid Dehydrogenase 249
32 Purification and Characterization of Recombinant Human Placental and Rat Lens Aldose Reductases Expressed in Escherichia coli 259
33 Rat and Human Bile Acid Binders Are Members of the Monomeric Reductase Gene Family 269
34 The Alcohol Dehydrogenase System 281
35 Promoters of the Mammalian Class III Alcohol Dehydrogenase Genes 295
36 Class I and Class IV Alcohol Dehydrogenase (Retinol Dehydrogenase) Gene Expression in Mouse Embryos 301
37 Molecular Evolution of Class I Alcohol Dehydrogenases in Primates: Models for Gene Evolution and Comparison of 3' Untranslated Regions of cDNAS 315
38 The Role of Leucine 116 in Determining Substrate Specificity in Human B[subscript 1] Alcohol Dehydrogenase 321
39 Mutations of Human Class III Alcohol Dehydrogenase 327
40 Human and Rat Class IV Alcohol Dehydrogenases: Correlations of Primary Structures with Enzymatic Properties
41 Cloning and Expression of a Human Stomach Alcohol Dehydrogenase Isozyme
42 Purification and Properties of Murine Corneal Alcohol Dehydrogenase: Evidence for Class IV ADH Properties 349
43 Mammalian Class VI Alcohol Dehydrogenase: Novel Types of the Rodent Enzymes 355
44 Crystallizations of Novel Forms of Alcohol Dehydrogenase 365
45 Human Sorbitol Dehydrogenase Gene: cDNA Sequence and Expression 373
46 Short-Chain Dehydrogenases/Reductases 383
47 Zinc Binding of Alcohol and Sorbitol Dehydrogenases 397
48 Horse Liver Alcohol Dehydrogenase-Catalyzed Aldehyde Oxidation: The Sequential Oxidation of Alcohols to Carboxylic Acids under NADH Recycling Conditions 407
49 Analytical Approaches to Alcohol Dehydrogenase Structures 417
50 Purification and Characterization of S-Formylglutathione Hydrolase from Human, Rat and Fish Tissues 427
51 Useful Mutants of Zymomonas Mobilis Alcohol Dehydrogenase-2 Obtained by the Use of Polymerase Chain Reaction Random Mutagenesis 435
Index 441
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