Epigenetics Protocols

by Trygve O. Tollefsbol (Editor)

Overview

Epigenetics is the science of the heritable changes in DNA or its associated proteins in gene sequence, except for mutations. Considered a nontraditional genetic process, research in epigenetics has offered much promise, for example, in the field of non-Medelian genetics. Tollefsbol (U. of Alabama) and contributors offer a wide array of leading-edge analytical methods and techniques suitable for studying fundamental biological processes and therapeutic interventions. Topics of the 22 papers include the analysis of chromatin remodeling, telomere position effects, quantitative epigenetics, and ADP ribosylation. They also include new techniques in DNA methylation and its relationship to chromatin structure. Chapters include methods, materials, instructions for study and application, and resources. Annotation ©2004 Book News, Inc., Portland, OR

Product Details

ISBN-13:	9781588293367
Publisher:	Springer-Verlag New York, LLC
Publication date:	07/23/2004
Series:	Methods in Molecular Biology , #287
Edition description:	2004
Pages:	304
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Methods of Epigenetic Analysis.- Chromatin Immunoprecipitation Assays.- Native Chromatin Immunoprecipitation.- Q-PCR in Combination With ChIP Assays to Detect Changes in Chromatin Acetylation.- Restriction Endonuclease Accessibility as a Determinant of Altered Chromatin Structure.- Measuring Changes in Chromatin Using Micrococcal Nuclease.- DNaseI Hypersensitivity Analysis of Chromatin Structure.- Inhibition of Histone Deacetylases.- Site-Specific Analysis of Histone Methylation and Acetylation.- Analysis of Mammalian Telomere Position Effect.- Activity Assays for Poly-ADP Ribose Polymerase.- Multigenerational Selection and Detection of Altered Histone Acetylation and Methylation Patterns.- Profiling DNA Methylation by Bisulfite Genomic Sequencing.- Methylation-Sensitive Single-Strand Conformation Analysis.- SIRPH Analysis.- Real-Time PCR-Based Assay for Quantitative Determination of Methylation Status.- Denaturing Gradient Gel Electrophoresis to Detect Methylation Changes in DNA.- Phorosslinking Oligonucleotide Hybridization Assay for Concurrent Gene Dosage and CpG Methylation Analysis.- Methylation-Specific Oligonucleotide Microarray.- Methylation-Specific PCR In Situ Hybridization.- Relative Quantitation of DNA Methyltransferase mRNA by Real-Time RT-PCR Assay.- DMB (DNMT-Magnetic Beads) Assay.

Editorial Reviews

"Comprehensive and easy to use...offers investigators readily reproducible techniques that will further promote progress in this critically important field." - Tumori

From the Publisher