Essential Cell Biology: A Practical Approach Volume 1: Cell Structure

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Biological and medical research relies upon an integrated understanding of the molecules within cells and of the interactions between cells. This has imposed great demands on investigators. Being an expert in a relatively narrow area is no longer sufficient as many studies now require the use of a wide range of techniques to provide the necessary integration. A lack of familiarity with the experimental possibilities can make such diversification difficult to achieve. This two volume set of Essential Cell Biology ...

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Overview

Biological and medical research relies upon an integrated understanding of the molecules within cells and of the interactions between cells. This has imposed great demands on investigators. Being an expert in a relatively narrow area is no longer sufficient as many studies now require the use of a wide range of techniques to provide the necessary integration. A lack of familiarity with the experimental possibilities can make such diversification difficult to achieve. This two volume set of Essential Cell Biology is designed to help researchers overcome these problems. It has not been possible to include all of the techniques available in cell biology so the challenge was to identify those that might be most relevant to researchers who are new to this topic. We have tried to cover both traditional and more recent approaches. The theory and basic principles of each technique are described, together with detailed protocols and advice for trouble shooting. Directions to more specialised techniques are also included. We hope the result inspires readers to experience the challenges and rewards of cell biology research for themselves and to contribute to the ongoing task of understanding the life of the cell.

Essential Cell Biology volume 1 focuses on techniques for studying cell structure whilst volume 2 concentrates on understanding how the cell functions. Volume 1 details the essential background information and protocols for observing and understanding cell morphology and cell structure, including, for example, investigations of nucleic acids, lipids, and the cytoskeleton. This is the essential guide to cell biology for researchers new to the field.

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Product Details

  • ISBN-13: 9780199638307
  • Publisher: Oxford University Press, USA
  • Publication date: 8/7/2003
  • Series: Practical Approach Series, #262
  • Pages: 424
  • Product dimensions: 9.80 (w) x 7.40 (h) x 1.10 (d)

Meet the Author

University of Warwick
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Table of Contents

List of protocols
Abbreviations
1 Cell culture 1
2 Basic light microscopy 35
3 Electron microscopy in cell biology 63
4 Subcellular fractionation 113
5 Plant cell biology 133
6 Protein purification 163
7 Gel electrophoresis of proteins 197
8 Biophysical methods in structural cell biology 269
9 Nucleic acids 293
10 Cell lipids: from isolation to functional dynamics 317
11 Extracellular matrix protocols for the study of complex phenotypes 349
12 The cytoskeleton 365
A1: List of suppliers 391
Index 397
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