Essential Stem Cell Methods

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This is a fast-moving field, and these detailed methods will help drive advances in stem cell research. The editors have hand selected step-by-step methods from researchers with extensive reputations and expertise.

This volume, as part of the Reliable Lab Solutions series, delivers busy researchers a handy, time-saving source for the best methods and protocols in stem cells.

• Provides powerful research opportunities for those interested in perusing work in pluripotent stem cells, disease modeling, and other aspects of basic stem cell research

• Refines, organizes and updates popular methods from flagship series, Methods in Enzymology

• Highlights top downloads, enhanced with author tips and tricks and pitfalls to avoid

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Product Details

  • ISBN-13: 9780123750617
  • Publisher: Elsevier Science
  • Publication date: 4/1/2009
  • Series: Reliable Lab Solutions Series
  • Edition description: New Edition
  • Pages: 630
  • Product dimensions: 7.00 (w) x 8.90 (h) x 1.10 (d)

Meet the Author

Robert Lanza, M.D. is currently Chief Scientific Officer at Advanced Cell Technology, and Adjunct Professor of Surgical Sciences at Wake Forest University School of Medicine. He has several hundred scientific publications and patents, and over 30 books, including Principles of Tissue Engineering (1st through 4th Editions), Methods of Tissue Engineering, Principles of Cloning (1st and 2nd Editions), Essentials of Stem Cell Biology (1st and 2nd Editions), XENO, Yearbook of Cell & Tissue Transplantation, One World: The Health & Survival of the Human Species in the 21st Century (as editor, with forewords by C. Everett Koop and former President Jimmy Carter), and Medical Science & the Advancement of World Health. Dr. Lanza received his B.A. and M.D. degrees from the University of Pennsylvania, where he was both a University Scholar and Benjamin Franklin Scholar. He is a former Fulbright Scholar, and studied as a student in the laboratory of Richard Hynes (MIT), Jonas Salk (The Salk Institute), and Nobel laureates Gerald Edelman (Rockefeller University) and Rodney Porter (Oxford University). He also worked closely (and coauthored a series of papers) with the late Harvard psychologist B.F. Skinner and heart transplant pioneer Christiaan Barnard. Dr. Lanza's current area of research focuses on the use of stem cells in regenerative medicine.

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Table of Contents

Section 1: Organ-Derived Stem Cells

1. Neural Stem Cell Isolation and Characterization
Rodney L. Rietze and Brent A. Reynolds
2. Neural Stem Cells and Their Manipulation Evan Snyder
3. Retinal Stem Cells
Thomas A. Reh and Andy J. Fischer
4. Dental Pulp Stem Cells
He Liu, Stan Gronthos and Songtao Shi
5. Spermatogonial Stem Cells
Jon M. Oatley and Ralph L. Brinster
6. Stem Cells in the Lung
Xiaoming Liu, Ryan R. Driskell and John F. Engelhardt
7. Pancreatic Cells and Their Progenitors
Seth J. Salpeter and Yuval Dor
8. Pluripotent Stem Cells from Germ Cells Candace L. Kerr, Michael J. Shamblott and John D. Gearhart
9. Amniotic Fluid and Placental Stem Cells Dawn M. Delo, Paolo De Coppi, Georg Bartsch, Jr. and Anthony Atala
10. Cord Blood Stem and Progenitor Cells Mervin C. Yoder
11. Purification of Hematopoietic Stem Cells Using the Side Population
K.K. Lin and Margaret A. Goodell
12. Microarray Analysis of Stem Cells and Differentiation
Howard Y. Chang
13. Tissue Engineering Using Adult Stem Cells Daniel Eberli
14. Mesenchymal Stem Cells and Tissue Engineering
Nicholas W. Marion and Jeremy J. Mao

Section II: Embryonic Stem Cells

15. Murine Embryonic Stem Cells
Andras Nagy and Kristina Vintersten
16. Human Embryo Culture
Amparo Mercader, Diana Valbuena and Carlos Simón
17. Human Embryonic Stem Cells
Douglas Melton
18. Characterization and Evaluation of Human Embryonic Stem Cells
Chunhui Xu
19. Feeder-Free Culture of Human Embryonic Stem Cells
Michal Amit and Joseph Itskovitz-Eldor
20. Neural Stem Cells, Neurons, and Glia
Steven M. Pollard, Alex Benchoua and Sally Lowell
21. Hematopoietic Cells
Malcolm A.S. Moore, Jae-Hung Shieh and Gabsang Lee
22. Cardiomyocytes
Xiangzhong Yang, Xi-Min Guo, Chang-Yong Wang and X. Cindy Tian
23. Insulin-Producing Cells
Insa S. Schroeder, Gabriela Kania, Przemyslaw Blyszczuk
24. Transgene Expression and RNA Interference in Embryonic Stem Cells
Holm Zaehres and George Q. Daley
25. Lentiviral Vector-Mediated Gene Delivery into Human Embryonic Stem Cells
Michal Gropp and Benjamin Reubinoff
26. Engineering Embryonic Stem Cells with Recombinase Systems
Frank Schnütgen, A. Francis Stewart, Harald von Melchner and Konstantinos Anastassiadis
27. Tissue Engineering Using Embryonic Stem Cells Shahar Cohen, Lucy Leshanski and Joseph Itskovitz-Eldor

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