Essential Zebrafish Methods: Cell and Developmental Biology

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Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development. This book, one of twonew volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biologyand updated by the original authorswhere relevant,it providesa comprehensive collection of protocols describing the mostwidely usedtechniques relevant to the study of the cellular and developmental biology of zebrafish. The methodsin thisvolume were hand-selected by the editors,whose goal was to a provide ahandy and cost-effectivecollection offail-safe methods, tips, and “tricks of the trade” to both experienced researchers and more junior members in the lab.

• Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors *Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error

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Product Details

  • ISBN-13: 9780123745996
  • Publisher: Elsevier Science
  • Publication date: 9/10/2009
  • Series: Reliable Lab Solutions Series
  • Pages: 584
  • Product dimensions: 7.40 (w) x 9.20 (h) x 1.10 (d)

Meet the Author

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

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Table of Contents

Overview of the Zebrafish System
H. William Detrich III
Cell Cycles and Development in the Embryonic Zebrafish
Donald A. Kane
Primary Fibroblast Cell Culture
Barry H. Paw
Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization)
John H. Postlethwait
Analysis of Protein and Gene Expression
Trevor Jowett
Analysis of Zebrafish Development Using Explant Culture Assays
Hazel Sive
Confocal Microscopic Analysis of Morphogenetic Movements
Mark S. Cooper
Cytoskeletal Dynamics of the Zebrafish Embryo
Lilianna Solinica-Krezel
Haploid Screens and Gamma-Ray Mutagenesis
Charline Walker
Positional Cloning of Mutated Zebrafish Genes
Will Talbot
The Zebrafish Genome
John Postlethwait
Analyzing Axon Guidance in the Zebrafish Retinotectal System
Chi-Bin Chien
Analysis of the Cell Cycle in Zebrafish Embryos
James F. Amatruda
Cellular Dissection of Zebrafish Hematopoiesis
David Traver
Culture of Embryonic Stem Cell Lines from Zebrafish
Paul Collodi
Laure Bally-Cuif
Time-Lapse Microscopy of Brain Development
Scott E. Fraser
Development of the Peripheral Sympathetic Nervous System in Zebrafish
Paul D. Henion
Approaches to Study Neurogenesis in the Zebrafish Retina
Jarema Malicki
Instrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms
David W. Tank
Development of Cartilage and Bone
Thomas F. Schilling
Morphogenesis of the Jaw: Development Beyond the Embryo
Pamela C. Yelick
Cardiac Development
Didier Y. R. Stainier
Zebrafish Kidney Development
Iain A. Drummond
Retroviral-Mediated Insertional Mutogenesis in Zebrafish
Adam Amsterdam
Genetic Screens for Maternal-Effect Mutations
Francisco Pelegri
Behavior Screening Assays in Zebrafish
Herwig Baier
A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish
Cecelia B. Moens
Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element
Koichi Kawakami
Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC)Probe Panel Resource for Genomic Analysis in Zebrafish
Charles Lee
The Zon Laboratory Guide to Positional Cloning in Zebrafish
Leonard I. Zon
Sleeping Beauty Transposon for Efficient Gene Delivery
Stephen C. Ecker
Transgene Manipulation in Zebrafish by Using Recombinases
Gary W. Stuart
Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI
Joachim Wittbrodt
Cloning Zebrafish by Nuclear Transfer
Shuo Lin
Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening
Christine Thisse
Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish
Bill Trevarrow
Common Diseases of Laboratory Zebrafish
Jennifer L. Matthews
Zebrafish Sperm Cryopreservation
Thomas Look

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