Extracellular Regulation Of Hedgehog And Wnt Signaling During Drosophila Wing Development.

Overview

Cell-bound members of the Heparan Sulfate Proteoglycan (HSPG) family such as such as Daily and Dlp are extracellular agents required for normal Hh activity. HSPGs are implicated during Hh transport through the tissues and in Hh reception. According to the prevailing view the physical affiliation between HSPGs and Hh requires a secreted protein encoded by shifted (shf). As expected the diffusion of tagged Hh is strongly reduced in shf mutants. Loss of Shf or HSPGs also leads to strong reductions in Hh ...
See more details below
This Paperback is Not Available through BN.com
Sending request ...

More About This Book

Overview

Cell-bound members of the Heparan Sulfate Proteoglycan (HSPG) family such as such as Daily and Dlp are extracellular agents required for normal Hh activity. HSPGs are implicated during Hh transport through the tissues and in Hh reception. According to the prevailing view the physical affiliation between HSPGs and Hh requires a secreted protein encoded by shifted (shf). As expected the diffusion of tagged Hh is strongly reduced in shf mutants. Loss of Shf or HSPGs also leads to strong reductions in Hh accumulation. Despite these advances several questions remain to be answered. Firstly, it is unclear whether normal Hh accumulation by Shf/HSPGs is a pre-requisite for normal Hh signaling. Secondly, despite the lack of shf or HSPGs, Hh continues to signal at short-range, suggesting that Hh movement is not completely perturbed, indicating that for Shf or HSPGs may be dispensable for Hh signaling at short-range. Thirdly, at high levels Hh does not require Shf to elicit response in target cells, suggesting that Hh may signal independently of Shf. In Chapter 1 we show that Dlp maintain short-range Hh signaling independently of Shf, indicating that Shf is dispensable for short-range Hh signaling. Interestingly, shf discs with raised Hh do not show corresponding increases in long-range Hh signaling. We provide evidence that implicates the role for Shf during Hh reception in areas where Hh levels are expected to be low. Finally we observe that increases in Hh signaling range does not require normal Hh accumulation and vise versa. We propose that normal Hh reception is more essential than normal Hh accumulation.

A vertebrate ortholog of Shf, the Wnt Inhibitory Factor-1 (WIF-1) has no known role in Hedgehog signaling. Instead, WIF-1 binds and inhibits vertebrate Wnts, and also inhibits Drosophila Wnt member Wingless (Wg), in all circumstances by an unknown mechanism. In contrast, Shf has no known role during Wg signaling. It remains in question whether WIF-1 and Shf have overlapping roles. Therefore we ask whether WIF-1 has a role in Hh signaling, and whether Shf may influence signaling by Wg. We find that HSPGs render WIFI-1 more effective in inhibiting Wnt/Wg in both vertebrates and flies. We also show that the vertebrate Wnt-binding WIF domain helps Hh signaling. Finally, we provide genetic evidence that implicates Shf involvement during Wg signaling. Together these findings reveal unexpected and overlapping roles between WIF-1 and Shf.

Read More Show Less

Product Details

  • ISBN-13: 9781244575936
  • Publisher: BiblioLabsII
  • Publication date: 9/30/2011
  • Pages: 154
  • Product dimensions: 7.44 (w) x 9.69 (h) x 0.33 (d)

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)