Fibrosis Research: Methods and Protocols / Edition 1

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Overview

Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it. In their discussion of research methodologies utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Editorial Reviews

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"...a must for the shelf of every researcher and physician interested in fibrotic diseases." -Rheumatology
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Product Details

  • ISBN-13: 9781588294791
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 8/28/2005
  • Series: Methods in Molecular Medicine Series , #117
  • Edition description: 2005
  • Edition number: 1
  • Pages: 392
  • Product dimensions: 6.20 (w) x 9.30 (h) x 1.10 (d)

Table of Contents

Part I. The Clinical Scope of Fibrosis

Pulmonary Fibrosis
David A. Zisman, Michael P. Keane, John A. Belperio, Robert M. Strieter, and Joseph P. Lynch, III

Renal Fibrosis
H. William Schnaper

Transforming Growth Factor-b: A Key Mediator of Fibrosis
Alain Mauviel

Part II. Regulation of Extracellular Matrix Metabolism: Cultured Cells

Isolation and Culture of Skin Fibroblasts
Laure Rittié and Gary J. Fisher

Isolation and Culture of Hepatic Stellate Cells
Ralf Weiskirchen and Axel M. Gressner

Isolation and Phenotypic Characterization of Lung Fibroblasts
Carolyn J. Baglole, Sireesha Y. Reddy, Stephen J. Pollock, Steven E. Feldon, Patricia J. Sime, Terry J. Smith, and Richard P. Phipps

Methods for Measuring Type I Collagen Synthesis In Vitro
David C. Rishikof, Ping-Ping Kuang, Mangalalaxmy Subramanian, and Ronald H. Goldstein

Methods for Assessing the Molecular Mechanisms Controlling Gene Regulation
Richard A. Rippe and Branko Stefanovic

Methods for Measuring TGF-b Using Antibodies, Cells, and Mice
Vladimir Jurukovski, Branka Dabovic, Vesna Todorovic, Yan Chen, and Daniel B. Rifkin

Part III. Studying Fibrosis Using Animal Models

Morphological Methods for Assessment of Fibrosis
Rakesh K. Kumar

Methods for Measuring Hydroxyproline and Estimating In Vivo Rates of Collagen Synthesis and Degradation
Robin J. McAnulty

Approaches to Evaluation of Fibrogenic Pathways in Surgical Lung Biopsy Specimens
Cory M. Hogaboam, Kristin J. Carpenter, Holly Evanoff, and Steven L. Kunkel

Animal Models for Adult Dermal Wound Healing
Mary Birch, Annette Tomlinson, and Mark W. J. Ferguson

Modeling Liver Fibrosis in Rodents
Christothea Constandinou, Neil Henderson, and John P. Iredale

Animal Models of Pulmonary Fibrosis
MehrnazGharaee-Kermani, Matthew Ullenbruch, and Sem H. Phan

Animal Models of Renal Fibrosis
Michael Zeisberg, Mary A. Soubasakos, and Raghu Kalluri

Animal Models of Cardiac Fibrosis
Yao Sun and Karl T. Weber

Part IV. Genetic Approaches to Studying Fibrosis

Finding Fibrosis Genes: The Lung
Lauranell H. Burch and David A. Schwartz

Genetic Studies to Identify Hepatic Fibrosis Genes and SNPs in Human Populations
Christopher Paul Day

Analysis of Microarray Experiments for Pulmonary Fibrosis
Nilesh B. Davé and Naftali Kaminski

DNA Microarrays and Data Mining to Study Hepatic Fibrosis
Bernd Schnabl, Youkyung Hwang Choi, Curt H. Hagedorn, and Ramón Bataller

Analysis of Proteins Dominantly Expressed in Hepatic Stellate Cells of Activated Phenotype: The Molecular Approach for Liver Fibrosis
Norifumi Kawada

Index

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