Fibrosis Research: Methods and Protocols / Edition 1

Fibrosis Research: Methods and Protocols / Edition 1

by John Varga
     
 

ISBN-10: 158829479X

ISBN-13: 9781588294791

Pub. Date: 08/28/2005

Publisher: Springer-Verlag New York, LLC

Fibrosis Research: Methods and Protocols contains a wealth of information concerning fibrosis research. Topics covered in the text include: ECM Regulation, Animal models of fibrosis, and the Genetic approaches to fibrosis.  See more details below

Overview

Fibrosis Research: Methods and Protocols contains a wealth of information concerning fibrosis research. Topics covered in the text include: ECM Regulation, Animal models of fibrosis, and the Genetic approaches to fibrosis.

Product Details

ISBN-13:
9781588294791
Publisher:
Springer-Verlag New York, LLC
Publication date:
08/28/2005
Series:
Methods in Molecular Medicine Series, #117
Edition description:
2005
Pages:
392
Product dimensions:
6.20(w) x 9.30(h) x 1.10(d)

Table of Contents

Part I. The Clinical Scope of Fibrosis

Pulmonary Fibrosis
David A. Zisman, Michael P. Keane, John A. Belperio, Robert M. Strieter, and Joseph P. Lynch, III

Renal Fibrosis
H. William Schnaper

Transforming Growth Factor-b: A Key Mediator of Fibrosis
Alain Mauviel

Part II. Regulation of Extracellular Matrix Metabolism: Cultured Cells

Isolation and Culture of Skin Fibroblasts
Laure Rittié and Gary J. Fisher

Isolation and Culture of Hepatic Stellate Cells
Ralf Weiskirchen and Axel M. Gressner

Isolation and Phenotypic Characterization of Lung Fibroblasts
Carolyn J. Baglole, Sireesha Y. Reddy, Stephen J. Pollock, Steven E. Feldon, Patricia J. Sime, Terry J. Smith, and Richard P. Phipps

Methods for Measuring Type I Collagen Synthesis In Vitro
David C. Rishikof, Ping-Ping Kuang, Mangalalaxmy Subramanian, and Ronald H. Goldstein

Methods for Assessing the Molecular Mechanisms Controlling Gene Regulation
Richard A. Rippe and Branko Stefanovic

Methods for Measuring TGF-b Using Antibodies, Cells, and Mice
Vladimir Jurukovski, Branka Dabovic, Vesna Todorovic, Yan Chen, and Daniel B. Rifkin

Part III. Studying Fibrosis Using Animal Models

Morphological Methods for Assessment of Fibrosis
Rakesh K. Kumar

Methods for Measuring Hydroxyproline and Estimating In Vivo Rates of Collagen Synthesis and Degradation
Robin J. McAnulty

Approaches to Evaluation of Fibrogenic Pathways in Surgical Lung Biopsy Specimens
Cory M. Hogaboam, Kristin J. Carpenter, Holly Evanoff, and Steven L. Kunkel

Animal Models for Adult Dermal Wound Healing
Mary Birch, Annette Tomlinson, and Mark W. J. Ferguson

Modeling Liver Fibrosis in Rodents
Christothea Constandinou, Neil Henderson, and John P. Iredale

Animal Models of Pulmonary Fibrosis
MehrnazGharaee-Kermani, Matthew Ullenbruch, and Sem H. Phan

Animal Models of Renal Fibrosis
Michael Zeisberg, Mary A. Soubasakos, and Raghu Kalluri

Animal Models of Cardiac Fibrosis
Yao Sun and Karl T. Weber

Part IV. Genetic Approaches to Studying Fibrosis

Finding Fibrosis Genes: The Lung
Lauranell H. Burch and David A. Schwartz

Genetic Studies to Identify Hepatic Fibrosis Genes and SNPs in Human Populations
Christopher Paul Day

Analysis of Microarray Experiments for Pulmonary Fibrosis
Nilesh B. Davé and Naftali Kaminski

DNA Microarrays and Data Mining to Study Hepatic Fibrosis
Bernd Schnabl, Youkyung Hwang Choi, Curt H. Hagedorn, and Ramón Bataller

Analysis of Proteins Dominantly Expressed in Hepatic Stellate Cells of Activated Phenotype: The Molecular Approach for Liver Fibrosis
Norifumi Kawada

Index

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