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Flourescence Microscopy of Living Cells in Culture, Part B: Quantitaive Flourescence Microscopy-Imaging and Spectroscopy

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Only recently has the true power of fluorescence techniques evolved for use with single living cells. The present status of the field reflects the occurrence of a revolution in cell biological research. Volume 30, along with Volume 29, provide the cell biologist with a sourcebook of methods. Volume 29 deals with the preparation, delivery, and detection of fluorescent probes. Volume 30 explores a combination of the theoretical and technical issues related to the quantitation of ...
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1989 Softcover 8vo, paperback, comb-bound. New. Bright, crisp & clean, unread; covers glossy. 503 pp.

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Overview

Only recently has the true power of fluorescence techniques evolved for use with single living cells. The present status of the field reflects the occurrence of a revolution in cell biological research. Volume 30, along with Volume 29, provide the cell biologist with a sourcebook of methods. Volume 29 deals with the preparation, delivery, and detection of fluorescent probes. Volume 30 explores a combination of the theoretical and technical issues related to the quantitation of fluorescence signals in the living cell with a light microscope.

Key Features
* Convenient bench-top format
Comprehensive methods included for:
* Imaging function, systems, and measurement
* Fluorescent standards and indicators
* Fluorescence ratio imaging, resonance energy transfer, and other microscopy methods
* Design of instruments and interpretation of data
* Applications to DNA, membranes, and other cellular components

Audience: Experienced cell biologists, biochemists, molecular biologists, and those specializing in instrumentation.

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Editorial Reviews

From the Publisher
"As with Volume 29, this book is clearly written and well edited. Some chapters cover techniques with a wide application in cell biology while others are more specialized. The two volumes should be of interest to many biologists and will make excellent reference books, being a useful addition to any library."
--BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
"The editors are to be commended for producing an excellent, informative reference work which will be of great use to all who are either using these techniques, or contemplating using them. All the contributors seem to have done their best to help and inform rather than to dazzle and intimidate. There are many hints and tips included that are not present in the original papers... I can thoroughly recommend these volumes to anyone who is interested in the problem of how to view the complex processes that take place in a living cell directly."
--TIBS
"Many experts have contributed to these volumes, which represent the first extensive overview of a rapidly developing new field of research. Quite a number of new techniques are investigated in depth by the contributors. The result is an overview that will not be outdated soon... It is certainly one of the merits of these volumes that they provide a nearly complete overview of the scientific and technical know-how required. One must congratulate the editors for having succeeded in convincing so many distinquished experts to contribute. The books are well printed with clear illustrations, and each volume has a good index."
--AMERICAN SCIENTIST
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Product Details

  • ISBN-13: 9780126847550
  • Publisher: Elsevier Science
  • Publication date: 11/28/1990
  • Series: Methods in Cell Biology Series
  • Edition description: REPRINT
  • Pages: 503

Table of Contents

I.T. Young, Image Fidelity. Characterizing the Imaging Transfer Function.
Z. Jericevic, B. Wiese, J. Bryan, and L.C. Smith, Validation of an Imaging System.
S. Inou, Imaging of Unresolved Objects, Superresolution, and Precision of Distance Measurement, with Video Microscopy.
J.E. Sisken, Fluorescent Standards.
R.Y. Tsien, Fluorescent Indicators of Ion Concentration.
G.R. Bright, G.W. Fisher, J. Rogowska, and D.L. Taylor, Fluorescence Ratio Imaging Microscopy.
D. Gross and L.M. Loew, Fluorescent Indicators of Membrane Potential: Microspectrofluorometry and Imaging.
B. Herman, Resonance Energy Transfer Microscopy.
D. Axelrod, Total Internal Reflection Fluorescence Microscopy.
D.E. Wolf, Designing, Building, and Using a Fluorescence Recovery after Photobleaching Instrument.
E.L. Elson and H. Qian, Interpretation of Fluorescence Correlation Spectroscopy and Photobleaching Recovery in Terms of Molecular Interactions.
D. Axelrod, Fluorescence Polarization Microscopy.
D.A. Agard, Y. Hiraoka, P. Shaw, and J.W. Sedat, Fluorescence Microscopy in Three Dimensions.
G.J. Brakenhoff, E.A. van Spronsen, H.T.M. Van der Voort, and N. Nanninga, Three-Dimensional Confocal Fluorescence Microscopy.
D. Axelrod and E.H. Hellen, Emission of Fluorescence at an Interface.
D.J. Arndt-Jovin and T.M. Jovin, Fluorescence Labeling and Microscopy of DNA.
A. Waggoner, R. DeBiasio, P. Conrad, G.R. Bright, L. Ernst, K. Ryan, M. Nederlof, and D. Taylor, Multiple Spectral Parameter Imaging. Each chapter includes references.
Index.
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