Flow Cytometry: Principles and Applications / Edition 1

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Overview

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers.

The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

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Editorial Reviews

From the Publisher
From the reviews:

"This book ‘Flow Cytometry: Principles and Applications’ focuses on flow cytometry as being an integral part of both basic biological research and clinical diagnosis in pathology. This volume provides a clear and practical manual especially for non-clinicians working in the clinical or experimental laboratory. … immunologists and haematologists in the field of research, as well as biological researchers working with both human and animal models, will appreciate this book." (Jan Philippé, Acta Clinica Belgica, Vol. 63 (2), 2008)

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Product Details

  • ISBN-13: 9781588296917
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 4/28/2007
  • Edition description: 2007
  • Edition number: 1
  • Pages: 294
  • Product dimensions: 6.10 (w) x 9.10 (h) x 0.90 (d)

Table of Contents


Preface     v
Contributors     ix
Principles of Flow Cytometry   Marion G. Macey     1
Cell Preparation   Desmond A. McCarthy     17
Fluorochromes and Fluorescence   Desmond A. McCarthy     59
Quality Control in Flow Cytometry   David Barnett   John T. Reilly     113
Experimental Design, Data Analysis, and Fluorescence Quantitation   Mark W. Lowdell     133
Apoptosis Detection by Flow Cytometry   Paul Allen   Derek Davies     147
DNA Analysis by Flow Cytometry   Derek Davies   Paul Allen     165
Immunological Studies of Human Cells   Ulrika Johansson     181
Calcium: Cytoplasmic, Mitochondrial, Endoplasmic Reticulum, and Flux Measurements   Gary Warnes   Marion G. Macey     209
Further Functional Studies   Marion G. Macey     219
Cell Sorting by Flow Cytometry   Derek Davies     257
Useful Internet Sites and Suppliers     277
Index     287
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