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Flow Cytometry, Part A

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Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow ...

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Overview

Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.

Key Features
• Completely revised and greatly expanded since the publication of the First Edition in 1990
• Methods cover cell death and cell cycle analyses
Practical, handbook-style presentation works in lab or classroom
• Unique comprehensive methodological coverage
• Color plates illustrate techniques
• In-depth treatment of procedures, including a description of each procedure:
• Theoretical foundations
• Critical aspects
• Possible pitfalls
• Written by authors with extensive experience who:
• Developed or modified the techniques
• Describe their experience with different instruments and applications to different cell systems
• Are the Who's Who in Flow Cytometry

Audience: Researchers, graduate students, and technicians in cell and developmental biology, pharmacology, toxicology, biochemistry, genetics and molecular biology, biotechnology, immunology, oncology, pathology, plant and marine science, and ecology.

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Editorial Reviews

From the Publisher
"The Second Edition of Flow Cytometry provides a comprehensive and detailed foundation in the application of this diverse and rapidly expanding technology. The variety and scope of topics covered in this two-volume set are too large to cover in detail. The second edition of Flow Cytometry provides the investigator with a concise compilation of new and updated applications and procedures, updated references and a directory of the principal contributors to the field. The additions and improvements to the Second Edition are evidence of the impact this technology will continue to have on biological and medical sciences for years to come. "
—AMERICAN SCIENTIST
"This is a good reference manual for a multi-user facility faced with a wide variety of biological applications."
—CYTOMETRY
"Flow Cytometry includes an impressive array of methods applicable to chromosome analysis, plant biology, marine biology, fluorescence, in situ hybridization, and others. It succeeds in providing the reader with good insight into the power of the technology throughout biology."
—KENNETH A. AULT, Maine Cytometry Research Institute, Maine Medical Center, in CANCER CELLS
"Every chapter has a clear introduction to the background of the method under discussion comprehensible to the inexperienced reader. Applications are very complete."
—B. Lambrecht-Ghent in CELL BIOLOGY INTERNATIONAL
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Product Details

  • ISBN-13: 9780125641425
  • Publisher: Elsevier Science
  • Publication date: 11/7/1994
  • Edition description: REV
  • Edition number: 2
  • Pages: 591

Meet the Author

John P. Robinson is a Professor of Sociology at the University of Maryland, College Park. Dr. Robinson founded and directed the Survey Research Center at the University of Maryland and the Communication Research Center at Cleveland State University. He has published more than 100 articles in professional journals and books and is a contributing editor to American Demographics magazine. Dr. Robinson was an American Statistical Association/National Science Foundation fellow at the Bureau of Labor Statistics in 1992-93, a Fulbright scholar at Moscow State University and Soviet Academy of Sciences in 1990, a Research Consultant at BBC News in 1978 and Research Coordinator for the U.S. Surgeon General’s Program on Television and Human Behavior in 1970. He received the 1987 Fordham University McGannon Award for Social and Ethical Relevance in Communication Policy Research for his research on improving public understanding of the news. His areas of specialization include social science methodology, attitude and behavior measurement, social change, and the impact of mass communication and other home technology. Additionally, he is an Editor of the Encyclopedia of Food Science, Food Technology & Nutrition.

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Table of Contents

D.W. Visscher and J.D. Crissman, Dissociation of Intact Cells from Tumors and Normal Tissues.
Z. Darzynkiewicz, X. Li, and J. Gong, Assays of Cell Viability: Discrimination of Cells Dying by Apoptosis.
C.C. Stewart and S.J Stewart, Cell Preparation for the Identification of Leukocytes.
C.C. Stewart and S.J. Stewart, Multiparameter Analysis of Leukocytes by Flow Cytometry.
G.F. Babcock and S.M. Dawes, Immunophenotyping Using Fixed Cells.
P.P.T.Brons, P.E.J. Van Erp, and A.H.M. Pennings, Simultaneous DNA Content and Cell-Surface Immunofluorescence Analysis.
H.M. Shapiro, Cell Membrane Potential Analysis.
M.J. Boyer and D.W. Hedley, Measurement of Intracellular pH.
C.H. June and P.S. Rabinovitch, Intracellular Ionized Calcium.
H.A. Crissman and J.A. Steinkamp, Cellular Protein Content Measurements.
F. Traganos and Z. Darzynkiewicz, Lysosomal Proton Pump Activity: Supravital Cell Staining with Acridine Orange Differentiates Leukocyte Subpopulations.
H.A. Crissman and G.T. Hirons, Staining of DNA in Live and Fixed Cells.
F.J. Otto, High-Resolution Analysis of Nuclear DNA Employing the Fluorochrome DAPI.
L.L. Vindeløv and I.J. Christensen, Detergent and Proteolytic Enzyme-Based Techniques for Nuclear Isolation and DNA Content Analysis.
D.W. Hedley, DNA Analysis from Paraffin-Embedded Blocks.
L.G. Dressler and L.C. Seamer, Controls, Standards, and Histogram Interpretation in DNA Flow Cytometry.
P.S. Rabinovitch, DNA Content Histogram and Cell-Cycle Analysis.
F. Dolbeare and J.R. Selden, Immunochemical Quantitation of Bromodeoxyuridine: Application to Cell-Cycle Kinetics.
J.A. Aten, J. Stap, R. Hoebe, and P.J.M. Bakker, Application and Detection of IdUrd and CldUrd as Two Independent Cell-Cycle Markers.
M. Poot, H. Hoehn, M. Kubbies, A. Grossmann, Y. Chen, and P.S. Rabinovitch, Cell-Cycle Analysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33358—Ethidium Bromide Bivariate Flow Cytometry.
H.A. Crissman, N. Oishi, and R. Habbersett, Detection of BrdUrd-Labeled Cells by Differential Fluorescence Analysis of DNA Fluorochromes: Pulse-Chase and Continuous Labeling Methods.
K.D. Bauer and J.W. Jacobberger, Analysis of Intracellular Proteins.
J.K. Larsen, "Washless" Procedures for Nuclear Antigen Detection.
W. Giaretti and M. Nüsse, Light Scatter of Isolated Cell Nuclei as a Parameter Discriminating the Cell-Cycle Subcompartments.
Z. Darzynkiewicz, Simultaneous Analysis of Cellular RNA and DNA Content.
Z. Darzynkiewicz, J. Gong, and F. Traganos, Analysis of DNA Content and Cyclin Protein Expression in Studies of DNA Ploidy, Growth Fraction, Lymphocyte Stimulation, and the Cell-Cycle.
J.P. Robinson, W.O. Carter, and P.K. Narayanan, Oxidative Product Formation Analysis by Flow Cytometry.
S. Klingel, G. Rothe, W. Kellermann, and G. Valet, Flow Cytometric Determination of Cysteine and Serine Proteinase Activities in Living Cells with Rhodamine 110 Substrates.
J.J. Turek and J.P. Robinson, Leucine Aminopeptidase Activity by Flow Cytometry.
J.V. Watson and C. Dive, Enzyme Kinetics.
K. Nooter, H. Herweijer, R.R. Jonker, and G.J. van den Engh, On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements.
Z. Darzynkiewicz, Acid-Induced Denaturation of DNA in Situ as a Probe of Chromatin Structure.
J. Laffin and J.M. Lehman, Detection of Intracellular Virus and Viral Products.
Index.
Volumes in Series.

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