Food-Borne Pathogens: Methods and Protocols / Edition 1

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More About This Textbook

Overview

A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Product Details

  • ISBN-13: 9781617375859
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/19/2010
  • Series: Methods in Biotechnology Series , #21
  • Edition description: Softcover reprint of hardcover 1st ed. 2006
  • Edition number: 1
  • Pages: 268
  • Product dimensions: 9.00 (w) x 6.00 (h) x 0.63 (d)

Table of Contents

Part I. The Bacteria

Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources: PCR Analysis and Biological Activity

Rosabel Falcón, Tatiana d'Albuquerque e Castro, Maria das Graças de Luna, Angela Corrêa de Freitas-Almeida, and Tomomasa Yano

Detection and Purification of Bacillus cereus Enterotoxins

Toril Lindbäck and Per Einar Granum

Campylobacter: Isolation, Identification, and Preservation

Rachel Gorman and Catherine C. Adley

Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces

Miia Lindström, Mari Nevas, and Hannu Korkeala

Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex

Peter C. H. Feng and Steven R. Monday

PulseNet's Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis

Lewis M. Graves and Balasubramanian Swaminathan

Plesiomonas shigelloides: Detection by PCR

Ivan Ciznar, Carlos González-Rey, Karel Krovacek, and Anna Hostacka

Pulsed-Field Gel Electrophoresis As a Molecular Technique in Salmonella Epidemiological Studies

Rachel Gorman and Catherine C. Adley

Kits for Detection of Food Poisoning Toxins Produced by Bacillus cereus and Staphylococcus aureus

Moira M. Brett

Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio cholerae From Food Samples

Keya De, Ranjan K. Nandy, and G. Balakrish Nair

HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes

Jennifer Colford

Part II. The Viruses

Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR

Christian M. Beuret

Detection of Enteroviruses

Miguel-Angel Jiménez-Clavero, Victoria Ley, Nuria Gómez, and Juan-Carlos Sáiz

Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification

Julie Jean and Ismaïl Fliss

Part III. The Parasites

Isolation and Characterization of Cathepsin-L1 Protease From Fasciola hepatica Excretory-Secretory Products for Serodiagnosis of Human Fasciolosis

Sandra M. O'Neill, Grace Mulcahy, and John P. Dalton

Molecular Biology Methods for Detection and Identification of Cryptosporidium Species in Feces, Water, and Shellfish

Colm J. Lowery, L. Xiao, U. M. Ryan, James S. G. Dooley, B. Cherie Millar, and John E. Moore

Molecular Identification of Nematode Worms From Seafood (Anisakis spp. and Pseudoterranova spp.) and Meat (Trichinella spp.)

Giuseppe La Rosa, Stefano D'Amelio, and Edoardo Pozio

Part IV. Parallel Studies to the Analysis of Food-Borne Pathogens

Approaches to Developing Quantitative Risk Assessment Models

Enda J. Cummins

A Review of Surveillance Networks of Food-Borne Diseases

Camelia Molnar, Rita Wels, and Catherine C. Adley

Index

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