Food-Borne Pathogens: Methods and Protocols / Edition 1

Paperback (Print)
Buy New
Buy New from
Used and New from Other Sellers
Used and New from Other Sellers
from $99.00
Usually ships in 1-2 business days
(Save 28%)
Other sellers (Paperback)
  • All (6) from $99.00   
  • New (5) from $99.00   
  • Used (1) from $181.20   

More About This Textbook


A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Read More Show Less

Product Details

  • ISBN-13: 9781617375859
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/19/2010
  • Series: Methods in Biotechnology Series, #21
  • Edition description: Softcover reprint of hardcover 1st ed. 2006
  • Edition number: 1
  • Pages: 268
  • Product dimensions: 9.00 (w) x 6.00 (h) x 0.63 (d)

Table of Contents

Part I. The Bacteria

Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources: PCR Analysis and Biological Activity

Rosabel Falcón, Tatiana d'Albuquerque e Castro, Maria das Graças de Luna, Angela Corrêa de Freitas-Almeida, and Tomomasa Yano

Detection and Purification of Bacillus cereus Enterotoxins

Toril Lindbäck and Per Einar Granum

Campylobacter: Isolation, Identification, and Preservation

Rachel Gorman and Catherine C. Adley

Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces

Miia Lindström, Mari Nevas, and Hannu Korkeala

Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex

Peter C. H. Feng and Steven R. Monday

PulseNet's Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis

Lewis M. Graves and Balasubramanian Swaminathan

Plesiomonas shigelloides: Detection by PCR

Ivan Ciznar, Carlos González-Rey, Karel Krovacek, and Anna Hostacka

Pulsed-Field Gel Electrophoresis As a Molecular Technique in Salmonella Epidemiological Studies

Rachel Gorman and Catherine C. Adley

Kits for Detection of Food Poisoning Toxins Produced by Bacillus cereus and Staphylococcus aureus

Moira M. Brett

Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio cholerae From Food Samples

Keya De, Ranjan K. Nandy, and G. Balakrish Nair

HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes

Jennifer Colford

Part II. The Viruses

Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR

Christian M. Beuret

Detection of Enteroviruses

Miguel-Angel Jiménez-Clavero, Victoria Ley, Nuria Gómez, and Juan-Carlos Sáiz

Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification

Julie Jean and Ismaïl Fliss

Part III. The Parasites

Isolation and Characterization of Cathepsin-L1 Protease From Fasciola hepatica Excretory-Secretory Products for Serodiagnosis of Human Fasciolosis

Sandra M. O'Neill, Grace Mulcahy, and John P. Dalton

Molecular Biology Methods for Detection and Identification of Cryptosporidium Species in Feces, Water, and Shellfish

Colm J. Lowery, L. Xiao, U. M. Ryan, James S. G. Dooley, B. Cherie Millar, and John E. Moore

Molecular Identification of Nematode Worms From Seafood (Anisakis spp. and Pseudoterranova spp.) and Meat (Trichinella spp.)

Giuseppe La Rosa, Stefano D'Amelio, and Edoardo Pozio

Part IV. Parallel Studies to the Analysis of Food-Borne Pathogens

Approaches to Developing Quantitative Risk Assessment Models

Enda J. Cummins

A Review of Surveillance Networks of Food-Borne Diseases

Camelia Molnar, Rita Wels, and Catherine C. Adley


Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)