Forensic Applications of High Performance Liquid Chromatography / Edition 1

Forensic Applications of High Performance Liquid Chromatography / Edition 1

by Shirley Bayne, Michelle Carlin
     
 

ISBN-10: 1420091913

ISBN-13: 9781420091915

Pub. Date: 01/15/2010

Publisher: Taylor & Francis

Chromatography has many roles in forensic science, ranging from toxicology to environmental analysis. In particular, high-performance liquid chromatography (HPLC) is a primary method of analysis in many types of laboratories. Maintaining a balance between practical solutions and the theoretical considerations involved in HPLC analysis, Forensic Applications

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Overview

Chromatography has many roles in forensic science, ranging from toxicology to environmental analysis. In particular, high-performance liquid chromatography (HPLC) is a primary method of analysis in many types of laboratories. Maintaining a balance between practical solutions and the theoretical considerations involved in HPLC analysis, Forensic Applications of High Performance Liquid Chromatography uses real-life examples likely to be found within a forensic science laboratory to explain HPLC from a forensic perspective.

Focusing chiefly on the reverse phase HPLC mode of separation, this volume examines:

  • The history of HPLC and the theory behind the separation process
  • The requirements for successful analysis and best practice tips
  • The modes of separation and detection most appropriate for forensic science applications
  • HPLC method development and evaluation
  • The quality aspects of laboratory operation
  • Troubleshooting HPLC systems and analyses
  • Applications of HPLC within the field of forensic science

Designed as a textbook for university students studying analytical chemistry, applied chemistry, forensic chemistry, or other courses with an element of HPLC within the course curriculum, this volume is also an invaluable guide for those in the early stages of their forensic analysis careers.

An instructor's manual with lecture slides, test bank, objectives, and exercises is available with qualifying course adoption

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Product Details

ISBN-13:
9781420091915
Publisher:
Taylor & Francis
Publication date:
01/15/2010
Series:
Analytical Concepts in Forensic Chemistry Series
Pages:
276
Product dimensions:
6.10(w) x 9.20(h) x 0.60(d)

Table of Contents

Introduction to HPLC

Introduction

The History of Chromatography

Basic Instrumentation

Further Reading

Basic Principles of HPLC

Theory of Chromatography

Chemical Bonding and Polarity

Mobile Phase Preparation, the Use of Buffers, and Sample Preparation

Mobile Phase Preparation

Buffers

Sample and Standard Preparation

Modes of Separation

Introduction

Reverse Phase HPLC

Ion Exchange

Normal Phase HPLC

Detection Systems

Introduction

Theory

UV/Vis Detectors versus Diode Array Detectors

Electrochemical Detectors

Mass Spectrometry

Method Development in Reversed Phase HPLC

Introduction

Sample Composition

Which Column Will Affect the Separation?

Choosing a Mobile Phase

Automated Method Development

Method Development Strategy

Method Development Worked Example

System Suitability

Introduction

System Suitability

System Performance

Chromatographic Suitability

Qualification, Validation, and Verification

Introduction

Valid Analytical Measurement

Estimation of Uncertainty of Measurement, Sources of Error, and Statistical Evaluation

Instrument Qualification

Method Validation

Method Verification

Quality Systems

Introduction

What Is Quality?

Introduction to Quality Assurance

How Do We Ensure Quality in Our Laboratories?

A Quality Management System

Accreditation

Auditing

Troubleshooting HPLC Systems

Introduction

Troubleshooting

Tool Kit and Spare Parts

Preventative Maintenance

Column Care

Forensic Applications of HPLC

Introduction

Drug Analysis

Toxicological Analysis

Colour Analysis

Analysis of Explosives

Food and the Environment

Glossary

Index

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