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Leading forensic experts from around the world describe in detail their time-proven methods for identity testing through DNA analysis. Their state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The book covers PCR- based test systems, the now widely used STR typing systems (complete with details of both manual and automated detection systems), repeat unit mapping (MVR), direct phase minisequencing, capillary electrophoresis, and direct blotting. Also fully discussed are the recovery of DNA from a wide range of sample types (blood, semen, skeletal remains, and saliva), species testing, sex determination, and mihondrial DNA testing.
This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types, analysis of DNA as single and multi-locus VNTR probes, PCR amplification of STR and other loci, and mihondrial sequencing. The expert scientists writing here-many from laboratories around the world-also discuss how to interpret the results in cases of unknown identity and disputed parentage.
The book contains black-and-white illustrations.
Recovery of High-Molecular-Weight DNA from Blood and Forensic Specimens
P. M. Schneider
Recovery of DNA for PCR Amplification from Blood and Forensic Samples Using a Chelating Resin
J. M. Willard, D. A. Lee, and M. M. Holland
PCR Analysis of DNA from Fresh and Decomposed Bodies and Skeletal Remains in Medicolegal Death Investigations
M. N. Hochmeister
PCR Analysis from Cigaret Butts, Postage Stamps, Envelope Sealing Flaps, and Other Saliva-Stained Material
M. N. Hochmeister, O. Rudin, and E. Ambach
Quantification of DNA by Slot-Blot Analysis
Rapid Assessment of PCR Product Quality and Quantity by Capillary Electrophoresis
J. M. Butler
Southern Blotting of Genomic DNA for DNA Profiling
Preparation and Use of Alkaline-Phosphatase-Conjugated Oligonucleotide Probes for Single-Locus and Multilocus VNTR Analysis
W. P. Childs, Michael B. T. Webb, and G. Rysiecki
Preparation and Use of 32P-Labeled Single-Locus VNTR Probes in Identity Testing
J. Henke and L. Henke
Statistical Methods Employed in Evaluation of Single-Locus Probe Results in Criminal Identity Cases
B. S. Weir.
Interpretation and Statistical Evaluation of Multilocus DNA Fingerprints in Paternity and Relationship Testing
G. Rysiecki and J. F. Y. Brookfield.
Overview of PCR-Based Systems in Identity Testing
An Introduction to PCR Primer Design and Optimization of Amplification Reactions
J. M. Robertson and J. Walsh-Weller.
Analysis of Amplified Fragment-Length Polymorphisms (VNTR/STR Loci) for Human Identity Testing
B. Budowle and R. C. Allen
Amplification of Short Tandem Repeat Loci Using PCR
Manual Electrophoretic Methods for Genotyping Amplified STR Loci
C. P. Phillips, A. Carrecedo, and M. V. Lareu.
Genotyping STR Loci Using an Automated DNA Sequencer
Separation of PCR Fragments by Means of Direct Blotting Electrophoresis
L. Henke and J. Henke.
Amplification and Sequencing of Mihondrial DNA in Forensic Casework
R. J. Steighner and M. Holland.
Minisatellite Variant Repeat Unit Mapping Using PCR (MVR-PCR)
D. L. Neil
Sex Determination by PCR Analysis of the X-Y Amelogenin Gene
Species Determination by Analysis of the Cyhrome b Gene
Use of the AmpliType PM + HLA DQA1 PCR Amplification and Typing Kits for Identity Testing
M. L. Baird.
The Use of Capillary Electrophoresis in Genotyping STR Loci
J. M. Butler.
Solid-Phase Minisequencing as a Tool to Detect DNA Polymorphism