Forensic DNA Typing: Biology, Technology, and Genetics of STR Markers / Edition 2

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Overview

Since the enormously successful first edition of Forensic DNA Typing was published, the Human Genome Project has published a draft sequence of the human genome and completed the “finished” reference sequence. The advent of modern DNA technology has resulted in the increased ability to perform human identity testing—desirable in a number of situations including the determination of perpetrators of violent crime such as murder and rape, resolving unestablished paternity, and identifying remains of missing persons or victims of mass disasters. The technology has been utilized in identifying remains from victims of the World Trade Center twin towers collapse following the terrorist attacks of September 11, 2001, the President Clinton-Monica Lewinsky scandal, and the identification of the remains in the Tomb of the Unknown Soldier. Indeed, our perceptions of history have been changed with DNA evidence that revealed Thomas Jefferson fathered a child by one of his slaves. This book examines the science of current forensic DNA typing methods by focusing on the biology, technology, and genetic interpretation of short tandem repeat (STR) markers, which encompass the most common forensic DNA analysis methods used today. Ten new chapters have been added to accommodate the explosion of new information since the turn of the century.

• The only book available that specifically covers detailed information on mitochondrial DNA and the Y chromosome
• Chapters cover the topic from introductory level right up to "cutting edge" research
• High-profile cases are addressed throughout the book, near the sections dealing with the science or issues behind these cases
• NEW TO THIS EDITION: D.N.A. Boxes—boxed "Data, Notes & Applications" sections throughout the book offer higher levels of detail on specific questions

Since the enormously successful first edition of Forensic DNA Typing was published, the Human Genome Project has published a draft sequence of the human genome and completed the "finished" reference sequence. The advent of modern DNA technology has resulted in the increased ability to perform human identity testing--desirable in a number of situations including the determination of perpetrators of violent crime such as murder and rape, resolving unestablished paternity, and identifying remains of missing persons or victims of mass disasters. The technology has been utilized in identifying remains from victims of the World Trade Center twin towers collapse following the terrorist attacks of September 11, 2001, the President Clinton-Monica Lewinsky scandal, and the identification of the remains in the Tomb of the Unknown Soldier. Indeed, our perceptions of history have been changed with DNA evidence that revealed Thomas Jefferson fathered a child by one of his slaves. This book examines the science of current forensic DNA typing methods by focusing on the biology, technology, and genetic interpretation of short tandem repeat (STR) markers, which encompass the most common forensic DNA analysis methods used today. Ten new chapters have been added to accommodate the explosion of new information since the turn of the century.The only book available that specifically covers detailed information on mitochondrial DNA and the Y chromosome

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Editorial Reviews

From the Publisher
“When they write the history of forensic science for our times, Forensic DNA Typing will be cited as the classic text on the subject.”—Richard Saferstein, Ph.D.

... the book is an exceptionally comprehensive reference, touching on every relevant aspect of current forensic DNA typing practice. - Journal of Forensic Sciences

Apart from coverage, the book is well edited, attractively laid out, and makes productive use of its four-color format. - Journal of Forensic Sciences

… very good book, and will serve many practitioners and students of forensic DNA typing as a single source reference. - Journal of Forensic Sciences

It is hard to think of a topic in forensic DNA typing that is not treated in the book. - Journal of Forensic Sciences

“This is a volume which contains something of value to all forensic scientists.” — Journal of Clinical Forensic Medicine

“[One] that should be purchased rather than borrowed . . .it is an outstanding guide to the subject.” – Journal of Clinical Forensic Medicine

…up to date every few years, it has the potential to become the standard reference, perhaps something. - Journal of Forensic Sciences

A final point is that the book is a phenomenal bargain in this day and age at around $80 a copy. - Journal of Forensic Sciences

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Product Details

  • ISBN-13: 9780121479527
  • Publisher: Elsevier Science
  • Publication date: 2/22/2005
  • Edition description: REV
  • Edition number: 2
  • Pages: 688
  • Product dimensions: 7.68 (w) x 9.96 (h) x 1.68 (d)

Meet the Author

John M. Butler is a NIST Fellow and Group Leader at the U.S. National Institute of Standards and Technology, Gaithersburg, Maryland. Dr. Butler earned his Ph.D. from the University of Virginia while doing DNA research in the FBI Laboratory’s Forensic Science Research Unit. He has over 100 publications in this field and is a frequent presenter on the topic of DNA typing.

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Table of Contents

Ch. 1: Overview & History of DNA Typing
Ch. 2: DNA Biology Review
Ch. 3: Sample Collection, DNA Extraction, and DNA Quantitation
Ch. 4: The Polymerase Chain Reaction (DNA Amplification)
Ch. 5: Commonly Used Short Tandem Repeat Markers and Commercial Kits
Ch. 6: Biology of STRs
Ch. 7: Forensic Issues
Ch. 8: Single Nucleotide Polymorphisms & Other Bi-allelic Markers
Ch. 9: Y-Chromosome DNA Testing
Ch. 10: Mitochondrial DNA Analysis
Ch. 11: Non-Human DNA and Microbial Forensics
Ch. 12: DNA Separation Methods
Ch. 13: DNA Detection Methods
Ch. 14: Instrumentation for STR Typing
Ch. 15: STR Genotyping Issues
Ch. 16: Laboratory Validation
Ch. 17: New Technologies, Automation, and Expert Systems
Ch. 18: CODIS and DNA Databases
Ch. 19: Basic Genetic Principles, Statistics, & Probability
Ch. 20: STR Population Database Analyses
Ch. 21: Profile Frequency Estimates, Likelihood Ratios & Source Attribution
Ch. 22: Approaches to Statistical Analysis of Mixtures and Degraded DNA
Ch. 23: Kinship and Parentage Testing
Ch. 24: Mass Disaster DNA Victim Identification

App I: Reported STR Alleles
App II: Population Data-STR Allele Frequencies
App. III: Suppliers of DNA Analysis Supplies
App. IV: DAB QA Standards
App. V: DAB Recommendations on Statistics
App. VI: NRC II Recommendations
App. VII: Example DNA Cases

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