Forensic DNA Typing Protocols / Edition 1

by Angel Carracedo

Overview

A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested prools for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.

Product Details

ISBN-13:	9781617374623
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/10/2010
Series:	Methods in Molecular Biology , #297
Edition description:	Softcover reprint of hardcover 1st ed. 2005
Pages:	280
Product dimensions:	5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

Forensic DNA-Typing Technologies: A Review Angel Carracedo and Paula Sánchez-Diz DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR Silvano Köchl, Harald Niederstätter, and Walther Parson A Real-Time PCR Prool to Determine the Number of Amelogenine (X-Y) Gene Copies From Forensic DNA Samples Antonio Alonso and Pablo Martín Species Determination: The Role and Use of the Cyhrome b Gene Adrian Linacre and James Chun-I Lee Constructing STR Mulitplex Assays John M. Butler Y Chromosome STR Typing Leonor Gusmão and Cíntia Alves Using Online Databases for Developing SNP Markers of Forensic Interest Christopher Phillips SNP Typing in Forensic Genetics: A Review Beatriz Sobrino and Angel Carracedo Genotyping SNPs With the LightCycler María Victoria Lareu and Clara Ruiz-Ponte Universal Tag Arrays in Forensic SNP Analysis Marie Allen and Anna-Maria Divne SNP Typing on the NanoChip Electronic Microarray Claus Børsting, Juan J. Sanchez, and Niels Morling Genotyping SNPs Using a UV-Pholeavable Oligonucleotide in MALDI-TOF MS Peter M. Vallone, Kristina Fahr, and Markus Kostrzewa Mihondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing Marie Allen and Hanna Andréasson SNaPshot Typing of Mihondrial DNA Coding Region Variants Antonio Salas, Beatriz Quintáns, and Vanesa Álvarez-Iglesias Typing of Y Chromosome SNPs With Multiplex PCR Methods Juan J. Sanchez, Claus Børsting, and Niels Morling Y Chromosome SNP Analysis Using Single-Base Extension: A Hierarchical Multiplex Design María Brión SNaPshot for Pharmacogenetics by Minisequencing Klaus Bender Methods for the Study of Ancient DNA Christine Keyser-Tracqui and BertrandLudes Prools for Ancient DNATyping Cristian Capelli and Frank Tschentscher Index