Forensic DNA Typing Protocols / Edition 1

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A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.

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Product Details

  • ISBN-13: 9781617374623
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/10/2010
  • Series: Methods in Molecular Biology Series, #297
  • Edition description: Softcover reprint of hardcover 1st ed. 2005
  • Edition number: 1
  • Pages: 280
  • Product dimensions: 9.00 (w) x 6.00 (h) x 0.66 (d)

Table of Contents

Forensic DNA-Typing Technologies: A Review

Angel Carracedo and Paula Sánchez-Diz

DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR

Silvano Köchl, Harald Niederstätter, and Walther Parson

A Real-Time PCR Prool to Determine the Number of Amelogenine (X-Y) Gene Copies From Forensic DNA Samples

Antonio Alonso and Pablo Martín

Species Determination: The Role and Use of the Cyhrome b Gene

Adrian Linacre and James Chun-I Lee

Constructing STR Mulitplex Assays

John M. Butler

Y Chromosome STR Typing

Leonor Gusmão and Cíntia Alves

Using Online Databases for Developing SNP Markers of Forensic Interest

Christopher Phillips

SNP Typing in Forensic Genetics: A Review

Beatriz Sobrino and Angel Carracedo

Genotyping SNPs With the LightCycler

María Victoria Lareu and Clara Ruiz-Ponte

Universal Tag Arrays in Forensic SNP Analysis

Marie Allen and Anna-Maria Divne

SNP Typing on the NanoChip Electronic Microarray

Claus Børsting, Juan J. Sanchez, and Niels Morling

Genotyping SNPs Using a UV-Pholeavable Oligonucleotide in MALDI-TOF MS

Peter M. Vallone, Kristina Fahr, and Markus Kostrzewa

Mihondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing

Marie Allen and Hanna Andréasson

SNaPshot Typing of Mihondrial DNA Coding Region Variants

Antonio Salas, Beatriz Quintáns, and Vanesa Álvarez-Iglesias

Typing of Y Chromosome SNPs With Multiplex PCR Methods

Juan J. Sanchez, Claus Børsting, and Niels Morling

Y Chromosome SNP Analysis Using Single-Base Extension: A Hierarchical Multiplex Design

María Brión

SNaPshot for Pharmacogenetics by Minisequencing

Klaus Bender

Methods for the Study of Ancient DNA

Christine Keyser-Tracqui and Bertrand Ludes

Prools for Ancient DNATyping

Cristian Capelli and Frank Tschentscher


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