Free Radicals: A Practical Approach / Edition 1

Free Radicals: A Practical Approach / Edition 1

by Kelly Punchard, N. a. Punchard
     
 

ISBN-10: 0199635595

ISBN-13: 9780199635597

Pub. Date: 08/28/1996

Publisher: Oxford University Press, USA

With the recognition that oxygen and nitrogen radicals are involved in normal cell metabolism, free radical research has begun to feature in most disciplines in the life sciences. Increasingly, their implication in a number of human disease processes is being accepted, altough conclusive evidence is lacking in many instances, partly due to the difficulties in

Overview

With the recognition that oxygen and nitrogen radicals are involved in normal cell metabolism, free radical research has begun to feature in most disciplines in the life sciences. Increasingly, their implication in a number of human disease processes is being accepted, altough conclusive evidence is lacking in many instances, partly due to the difficulties in detecting and measuring free radicals that have lifetimes measured in microseconds. This book outlines the latest techniques for quantifying radicals and their effects, and features detailed protocols, hints, and tips for success, troubleshooting comments, sample data, and key literature citations.

Product Details

ISBN-13:
9780199635597
Publisher:
Oxford University Press, USA
Publication date:
08/28/1996
Series:
Practical Approach Series, #168
Edition description:
New Edition
Pages:
336
Product dimensions:
6.13(w) x 9.19(h) x 0.67(d)

Table of Contents

1. Introduction
2. Direct and indirect measurements of free radicals by physico-chemical methods
2.1. In vitro and in vivo detection of free radical metabolites with electron spin resonance
2.2. The application of high-field nuclear magnetic resonance spectroscopy to the detiction of products arising from the reactions of free radicals in chemical model systems, biosystems, and foodstuffs
2.3. Pulse radiolysis
3. Direct and indirect measurement of free radicals by other methods
3.1. Intrinsic (low-level) chemiluminescence
3.2. Visual assessment of oxidative stress by multifunctional digital microfluorography
3.3. Salicylic acid and phenylalanine as probes to detect hydroxyl radicals
4. Measurement of free radical products
4.1. Lipids (i): peroxides and other products
4.2. Lipids (ii): quantitative analysis of 4-hydroxyl 2-nonenal
4.3. Lipids (iii): F2-isoprostanes: prostaglandin-like products of lipik peroxidation
4.4. Proteins (i): determination of carbonyl groups in oxidized proteins
4.5. Proteins (ii): protein hydroperoxides, protein hydroxides, and protein-bound DOPA
4.6. Carbohydrates: investigating the effects of oxygen free radicals and carbohydrates in biological systems
4.7. Nucleic acids: measurements of products of free radical attack on nucleic acids
5. Measurement of antioxidants
5.1. Glutahione
5.2. Glutathione peroxidase: activity and steady-state level of mRNA
5.3. Superoxide dismutase
5.4. Application of deuterium labelling and gas chromatographt-mass spectrometry to the measurement of vitamin E bioavailability in tissue and body fluids
5.5. Selective and sensitive measurement of vitamin C, ubiquinol-10, and other low molecular weight antioxidants
5.6. Measurement of antioxidant gene expression

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