Description: This manual from the Practical Approach series is a compilation of techniques in free radical research as contributed by 44 contributors from numerous disciplines. It covers physicochemical methods of free radical detection, biochemical methods of detection, measurement of free radical products, and measurements of antioxidants, including measurement of antioxidant gene expression.
Purpose: This manual is meant to provide detailed practical knowledge of fundamental as well as newer techniques to those new to free radical research. This is especially of value because of the rapid expansion of our knowledge of the role of free radicals in normal cell metabolism and human diseases. Although this is a methods book, it would have been useful to provide, as an introduction, a broader overview of known functions of free-radicals in cell metabolism, especially in terms of signal transduction.
Audience: The audience is meant to be primarily biochemists and secondarily molecular biologists and cell biologists.
Features: This book has the typical attractive and very easy-to-read format of the Practical Approach series. It features at least several abbreviated protocols within each chapter as well as a list of suppliers at the end of the book.
Assessment: This book is highly recommended for the institutional as well as personal library because of the ubiquitous nature of free radical research. A manual on methods was sorely needed in this area and this book will be very useful.