From Genes to Genomes / Edition 2

From Genes to Genomes / Edition 2

by Jeremy W. Dale, Malcolm von Schantz
     
 

From Genes to Genomes: Concepts and Applications of DNA Technology, Second Edition
Jeremy W. Dale and Malcolm von Schantz, University of Surrey, UK.

Rapid advances in a collection of techniques referred to as gene technology, genetic engineering, recombinant DNA technology and gene cloning have pushed molecular biology to the forefront of

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Overview

From Genes to Genomes: Concepts and Applications of DNA Technology, Second Edition
Jeremy W. Dale and Malcolm von Schantz, University of Surrey, UK.

Rapid advances in a collection of techniques referred to as gene technology, genetic engineering, recombinant DNA technology and gene cloning have pushed molecular biology to the forefront of the biological sciences. From Genes to Genomes: Concepts and Applications of DNA Technology, Second Edition explains key ideas underlying the most central techniques in the context of the ways in which they are used. As well as an overall update of the material, this new edition includes the strengthening of the gene to genome theme, additional emphasis on proteomics, gene therapy and stem cells, and more eukaryotic/mammalian examples.

Opening with a brief review of the basic concepts of molecular biology, followed by a description of the key molecular methods and how they fit together, the book progresses from the cloning, characterisation and manipulation of individual genes through to genome sequence and analysis, and the genome-wide study of transcription and translation. Finally, the book introduces the use of these techniques for genetic modification of organisms, ranging from live vaccines to transgenic plants and animals, including the prospects for gene therapy.

  • An accessible introduction to the key concepts and techniques in an exciting and rapidly developing field.
  • Includes basic concepts as well as the latest developments.
  • Introduces selected aspects of bioinformatics.
  • Clear presentation with two-colour design throughout.

The new edition of this concise, clearly written textbook will prove invaluable to those studying intermediate molecular genetics within the biological and biomedical sciences as well as established researchers needing to update their knowledge of this fast moving field.

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Product Details

ISBN-13:
9780470017340
Publisher:
Wiley
Publication date:
12/04/2007
Edition description:
Reprint
Pages:
394
Product dimensions:
6.73(w) x 9.72(h) x 0.78(d)

Table of Contents

Preface.

1 Introduction.

2 Basic molecular biology.

2.1 Nucleic acid structure.

2.2 What is a gene?

2.3 Information flow: gene expression.

2.4 Gene structure and organization.

3 How to clone a gene.

3.1 What is cloning?

3.2 Overview of the procedures.

3.3 Gene libraries.

3.4 Hybridization.

3.5 Polymerase chain reaction.

3.6 Extraction and purification of nucleic acids.

3.7 Detection and quantitation of nucleic acids.

3.8 Gel electrophoresis.

4 Cutting and joining DNA.

4.1 Restriction endonucleases.

4.2 Ligation.

4.3 Modification of restriction fragment ends.

4.4 Other ways of joining DNA molecules.

5 Vectors.

5.1 Plasmid vectors.

5.2 Vectors based on the lambda bacteriophage.

5.3 Cosmids.

5.4 M13 vectors.

5.5 Expression vectors.

5.6 Vectors for cloning and expression in eukaryotic cells.

5.7 Supervectors: YACs and BACs.

5.8 Summary.

6 Genomic and cDNA libraries.

6.1 Genomic libraries.

6.2 Growing and storing libraries.

6.3 cDNA libraries.

6.4 Random, arrayed and ordered libraries.

7 Finding the right clone.

7.1 Screening libraries with gene probes.

7.2 Screening expression libraries with antibodies.

7.3 Subcloning.

7.4 Characterization of plasmid clones.

8 Polymerase chain reaction.

8.1 The PCR reaction.

8.2 PCR in practice.

8.3 Cloning PCR products.

8.4 Long-range PCR.

8.5 Reverse-transcription PCR.

8.6 Rapid amplification of cDNA ends.

8.7 Quantitative PCR.

8.8 Applications of PCR.

9 Characterization of a cloned gene.

9.1 DNA sequencing.

9.2 Databank entries and annotation.

9.3 Sequence analysis.

9.4 Sequence comparisons.

9.5 Protein structure.

9.6 Confirming gene function.

10 Analysis of gene expression.

10.1 Analysing transcription.

10.2 Methods for studying the promoter.

10.3 Regulatory elements and DNA-binding proteins.

10.4 Translational analysis.

11 Products from native and manipulated cloned genes.

11.1 Factors affecting expression of cloned genes.

11.2 Expression of cloned genes in bacteria.

11.3 Expression in eukaryotic host cells.

11.4 Adding tags and signals.

11.5 In vitro mutagenesis.

11.6 Vaccines.

12 Genomic analysis.

12.1 Genome sequencing.

12.2 Analysis and annotation.

12.3 Comparing genomes.

12.4 Genome browsers.

12.5 Relating genes and functions: genetic and physical maps.

12.6 Transposon mutagenesis and other screening techniques.

12.7 Conclusion.

13 Analysis of genetic variation.

13.1 Single nucleotide polymorphisms.

13.2 Larger-scale variations.

13.3 Other methods for studying variation.

13.4 Human genetic diseases.

13.5 Molecular phylogeny.

14 Post-genomic analysis.

14.1 Analysing transcription; transcriptomes.

14.2 Array-based methods.

14.3 Translational analysis; proteomics.

14.4 Post-translational analysis: protein interactions.

14.5 Integrative studies; systems biology.

15 Modifying organisms; transgenics.

15.1 Modification of bacteria and viruses: live vaccines.

15.2 Transgenesis and cloning.

15.3 Animal transgenesis.

15.4 Applications of transgenic animals.

15.5 Transgenic plants and their applications.

Glossary.

Bibliography.

Index.

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