Fundamentals of Light Microscopy and Electronic Imaging / Edition 1

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Overview

This practical book presents the fundamentals of light and electron microscopy, including the basics of microscope design, image formation, and camera function. Moreover, researchers will learn how to acquire electronic images and perform image processing. The author provides instructions on the latest techniques involving video microscopy, digital microscopy, laser scanning microscopy, digital CCD microscopy, as well as a chapter covering issues related to image processing for scientific publication.
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Editorial Reviews

From the Publisher
“This should be provided to all beginning graduate students entering microscopy labs. It describes the complicated hardware of the system, while also explaining the physics principles of microscopy on a simplistic level for basic biologists. The authors achieve a perfect balance of theory and methods.” (Doody’s, 15 November 2013)

“It should be particularly useful to researchers getting started in the field of microscopy as well as seasoned professionals. Summing Up: Highly recommended. Graduate students, researchers/faculty, and professionals/practitioners.” (Choice, 1 October 2013)

“In summary, Fundamentals of Light Microscopy, Second Edition is a recommended starting point for the novice in microscopy and electronic imaging.” (Journal of Biomedical Optics, 1 February 2013)

Doody's Review Service
Reviewer: Latha Malaiyandi, PhD (Midwestern University)
Description: This is a comprehensive overview of the new technology in light microscopy and electronic imaging introduced in the decade since the first edition was published in 2001.
Purpose: The goal is to provide cell biologists with a foundational basis in the tools and techniques required to image specimens of organisms and their components. The book starts with an introduction to fundamental principles of microscopy and each chapter adds in complexity to provide readers with more specific applications that may be relevant to their specific research goals.
Audience: The intended audience ranges from amateur microscopists to seasoned researchers who are looking to add sophisticated applications to their microscopy experiments.
Features: The book details the theoretical and practical knowledge of microscopy. It starts with basic light microscopy, but then delves into epi-fluorescence, confocal, and two-photon microscopy. It includes detailed photographs of internal microscopy hardware, beautiful examples of microscopy images of various specimens ranging from bacteria to mammalian tissues to whole organisms, such as Drosophila. Each chapter includes a list of recommended reviews on the topic. The diagrams and tables are well defined and provide an extensive comparison among different techniques, reagents, and instruments. The authors provide useful guidelines for selecting microscopes and microscope components based on user requirements without endorsing any one brand. In addition, they provide demonstrations of the care and maintenance of the instrument as well as useful applications (e.g. step-by-step instructions for calibrating a stage micrometer or aligning a mercury arc lamp). This new edition features exercises with answer keys, the materials and reagents needed for demonstrations, and an extensive list of web resources on microscopy.
Assessment: This should be provided to all beginning graduate students entering microscopy labs. It describes the complicated hardware of the system, while also explaining the physics principles of microscopy on a simplistic level for basic biologists. The authors achieve a perfect balance of theory and methods. Specifically, they discuss the theory behind microscopy, introduce practical applications, provide a historical basis for its development, and include their expert advice on the testing and purchasing of a microscopy system based on specific user requirements.

5 Stars! from Doody
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Product Details

  • ISBN-13: 9780471253914
  • Publisher: Wiley, John & Sons, Incorporated
  • Publication date: 12/28/2001
  • Edition description: Older Edition
  • Edition number: 1
  • Pages: 388
  • Product dimensions: 7.17 (w) x 9.98 (h) x 0.87 (d)

Meet the Author

DOUGLAS B. MURPHY supervises core facilities in microscopy and histology at the new HHMI Janelia Farm Research Campus in Ashburn, Virginia. An Adjunct Professor of Cell Biology at Johns Hopkins School of Medicine in Baltimore, Maryland, Dr. Murphy helped establish the School of Medicine Microscope Facility there, which he supervised until 2006.

MICHAEL W. DAVIDSON is an assistant scholar/scientist affiliated with the National High Magnetic Field Laboratory and the Department of Biological Science at Florida State University where he is involved in developing educational websites. His digital images and photomicrographs have graced the covers of over 2,000 publications.

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Table of Contents

Preface
1 Fundamentals of Light Microscopy 1
2 Light and Color 15
3 Illuminators, Filters, and Isolation of Specific Wavelengths 29
4 Lenses and Geometrical Optics 43
5 Diffraction and Interference in Image Formation 61
6 Diffraction and Spatial Resolution 85
7 Phase Contrast Microscopy and Dark-Field Microscopy 97
8 Properties of Polarized Light 117
9 Polarization Microscopy 135
10 Differential Interference Contrast (DIC) Microscopy and Modulation Contrast Microscopy 153
11 Fluorescence Microscopy 177
12 Confocal Laser Scanning Microscopy 205
13 Video Microscopy 233
14 Digital CCD Microscopy 259
15 Digital Image Processing 283
16 Image Processing for Scientific Publication 307
App. I 317
App. II 321
App. III 329
Glossary 331
References 357
Index 361
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Sort by: Showing 1 Customer Reviews
  • Anonymous

    Posted September 18, 2002

    Recommended Book

    "Both theoretical and practical aspects of the subject are treated with economy, clarity, and authority....Murphy¿s book is unusual because it provides a technically sound and simply conceived introduction to virtually every link in the chain of technologies that makes modern imaging so powerful....This book will provide individuals without background knowledge in optical physics, electronics, or image processing with many of the basic facts they need to know to understand both the power and the limitations of their images." --J. Richard McIntosh, Cell Biology Education

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