G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols

Overview

Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins. Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid ...

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Overview

Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins. Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and dependable, G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.

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Product Details

  • ISBN-13: 9781627035316
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 8/31/2013
  • Series: Methods in Molecular Biology Series , #1043
  • Edition description: 2013
  • Edition number: 1
  • Pages: 162
  • Product dimensions: 7.33 (w) x 10.09 (h) x 0.60 (d)

Table of Contents

1. Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs

Timothy E. Gookin and Jannick D. Bendtsen

2. Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins

Swarup Roy Choudhury, Corey S. Westfall, Dieter Hackenberg, and Sona Pandey

3. Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana

Jian Zhao and Xuemin Wang

4. Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants

Zhaoqing Jin, Wellington Muchero, and Jin-Gui Chen

5. Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G Proteins in Arabidopsis

Kiwamu Tanaka, Jeongmin Choi, and Gary Stacey

6. Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis

Guojing Li, Xiujuan Zhang, Dongli Wan, Shuqun Zhang, and Yiji Xia

7. Fusarium oxysporum Infection Assays in Arabidopsis

Yuri Trusov, David Chakravorty, and Jose Ramon Botella

8. Analysis of Unfolded Protein Response in Arabidopsis

Yani Chen and Federica Brandizzi

9. Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis

Wenjuan Wu and Jirong Huang

10. G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings

Danielle A. Orozco-Nunnelly, Lon S. Kaufman, and Katherine M. Warpeha

11. Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana

Xingyun Qi and Huanquan Zheng

12. In Vivo Localization in Arabidopsis Protoplasts and Root Tissue

Myoung Hui Lee, Yongjik Lee, and Inhwan Hwang

13. Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography–Coupled Mass Spectrometry

Nadav Sorek, Amir Akerman, and Shaul Yalovsky

14. In Vitro Myristoylation Assay of Arabidopsis Proteins

Xuehui Feng, Wan Shi, Xuejun Wang, and Mark P. Running

15. Assaying Protein S-Acylation in Plants

Piers A. Hemsley

16. In Vitro Prenylation Assay of Arabidopsis Proteins

Wan Shi, Qin Zeng, and Mark P. Running

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