G Protein-Coupled Receptors : Essential Methods / Edition 1

Hardcover (Print)
Used and New from Other Sellers
Used and New from Other Sellers
from $96.01
Usually ships in 1-2 business days
(Save 22%)
Other sellers (Hardcover)
  • All (5) from $96.01   
  • New (4) from $96.01   
  • Used (1) from $98.55   
Close
Sort by
Page 1 of 1
Showing All
Note: Marketplace items are not eligible for any BN.com coupons and promotions
$96.01
Seller since 2008

Feedback rating:

(17874)

Condition:

New — never opened or used in original packaging.

Like New — packaging may have been opened. A "Like New" item is suitable to give as a gift.

Very Good — may have minor signs of wear on packaging but item works perfectly and has no damage.

Good — item is in good condition but packaging may have signs of shelf wear/aging or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Acceptable — item is in working order but may show signs of wear such as scratches or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Used — An item that has been opened and may show signs of wear. All specific defects should be noted in the Comments section associated with each item.

Refurbished — A used item that has been renewed or updated and verified to be in proper working condition. Not necessarily completed by the original manufacturer.

New
Brand New, Perfect Condition, Please allow 4-14 business days for delivery. 100% Money Back Guarantee, Over 1,000,000 customers served.

Ships from: Westminster, MD

Usually ships in 1-2 business days

  • Canadian
  • International
  • Standard, 48 States
  • Standard (AK, HI)
$96.02
Seller since 2008

Feedback rating:

(4535)

Condition: New
New Book. Shipped from UK within 10 to 14 business days. Established seller since 2000.

Ships from: Horcott Rd, Fairford, United Kingdom

Usually ships in 1-2 business days

  • Standard, 48 States
  • Standard (AK, HI)
$99.11
Seller since 2007

Feedback rating:

(23585)

Condition: New
BRAND NEW

Ships from: Avenel, NJ

Usually ships in 1-2 business days

  • Canadian
  • International
  • Standard, 48 States
  • Standard (AK, HI)
$100.36
Seller since 2009

Feedback rating:

(10666)

Condition: New
New Book. Shipped from US within 10 to 14 business days. Established seller since 2000.

Ships from: Secaucus, NJ

Usually ships in 1-2 business days

  • Standard, 48 States
  • Standard (AK, HI)
Page 1 of 1
Showing All
Close
Sort by

More About This Textbook

Overview

G Protein-Coupled Receptors (GPCRs) are not only the largest protein family in the human genome but are also the single biggest target for therapeutic agents. Research into GPCRs is therefore growing at a fast pace and the range of techniques that can be applied to GPCRs is vast and continues to grow. This book provides an invaluable bench-side guide into the best and most up-to-date techniques for current and future research on GPCRs.

With contributions from leading international authorities, GPCR Essential Methods equips readers with clear and detailed protocols for both well-known and up-and-coming techniques along with hints and tips for success. All the methods have been tried and tested by leading international research labs and are presented in easy-to-follow stages along with a useful overview of each technique.

This book will be of interest to researchers in molecular biology, biochemistry, pharmacology and will be a useful resource for graduate students.

Highlights the best and most up-to-date techniques for working on GPCRs from the vast range currently in use

Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches

Chapters are written by leading international authorities in the field and cover both new and well-known, tried and tested methods for working with GPCRs

An essential laboratory manual for researchers at all levels

Read More Show Less

Product Details

  • ISBN-13: 9780470749142
  • Publisher: Wiley
  • Publication date: 2/9/2010
  • Edition number: 1
  • Pages: 310
  • Product dimensions: 6.80 (w) x 9.80 (h) x 0.90 (d)

Table of Contents

Preface ix

Contributors xi

1 Measurement of Ligand-G Protein-coupled Receptor Interactions Katie Leach Celine Valant Patrick M. Sexton Arthur Christopoulos 1

1.1 Introduction 1

1.2 Methods and approaches 5

References 27

2 Second Messenger Assays for G Protein-coupled Receptors: cAMP, Ca2+, Inositol Phosphates, ERK1/2 Karen J. Gregory Patrick M. Sexton Arthur Christopoulos Caroline A. Hick 31

2.1 Introduction 31

2.2 Methods and approaches 33

2.3 Troubleshooting 49

References 50

3 Use of the [35S]GTPγS Binding Assay to Determine Ligand Efficacy at G Protein-coupled Receptors Elodie Kara Philip G. Strange 53

3.1 Introduction 53

3.2 Methods and approaches 54

3.3 Troubleshooting 66

Acknowledgements 67

References 67

4 Quantitative Imaging of Receptor Trafficking Andy R. James Takeo Awaji F. Anne Stephenson Nicholas A. Hartell 69

4.1 Introduction 69

4.2 Methods and approaches 70

4.3 Troubleshooting 80

References 82

5 Production of Recombinant G Protein-coupled Receptor in Yeast for Structural and Functional Analysis Richard A.J. Darby Mohammed Jamshad Ljuban Grgic William J. Holmes Roslyn M. Bill 85

5.1 Introduction 85

5.2 Methods and approaches 86

5.3 Troubleshooting 106

References 107

6 Monitoring GPCR-Protein Complexes Using Bioluminescence Resonance Energy Transfer Werner C. Jaeger Kevin D.G. Pfleger Karin A. Eidne 111

6.1 Introduction 111

6.2 Methods and approaches 114

6.3 Troubleshooting 128

References 128

7 Using Intramolecular Fluorescence Resonance Energy Transfer to Study Receptor Conformation Cornelius Krasel Carsten Hoffmann 133

7.1 Introduction 133

7.2 Methods and approaches 135

7.3 Troubleshooting 143

References 144

8 A Disulfide Cross-linking Strategy Useful for Studying Ligand-induced Structural Changes in GPCRs Jian Hua Li Stuart D.C. Ward Sung-Jun Han Fadi F. Hamdan Jürgen Wess 147

8.1 Introduction 147

8.2 Methods and approaches 149

8.3 General considerations, caveats and troubleshooting 162

Acknowledgements 164

References 164

9 Use of Fluorescence Correlation Spectroscopy to Study the Diffusion of G Protein-coupled Receptors Stephen J. Briddon Jonathan A. Hern Stephen J. Hill 169

9.1 Introduction 169

9.2 Methods and approaches 170

9.3 Troubleshooting 190

References 191

10 Identification and Analysis of GPCR Phosphorylation Kok Choi Kong Sharad C. Mistry Andrew B. Tobin 197

10.1 Introduction 197

10.2 Methods 198

Acknowledgements 213

References 213

11 Measurement and Visualization of G Protein-coupled Receptor Trafficking by Enzyme-linked Immunosorbent Assay and Immunofluorescence Stuart J. Mundell Shaista P. Nisar Eamonn Kelly 215

11.1 Introduction 215

11.2 Methods and approaches 217

11.3 Troubleshooting 226

References 227

12 Substituted Cysteine Accessibility Method (SCAM) George Liapakis Jonathan A. Javitch 229

12.1 Introduction 229

12.2 Methods and approaches 230

12.3 Troubleshooting 247

References 248

13 Homology Modelling of G Protein-coupled Receptors John Simms 251

13.1 Introduction 252

13.2 Methods and approaches 252

13.3 Troubleshooting 269

13.4 Automated methods for generating models of GPCRs 269

References 270

Appendix Site-directed Mutagenesis and Chimeras Alex Conner Mark Wheatley David R. Poyner 275

A.1 Introduction 275

A.2 Why mutagenesis? 275

A.3 Troubleshooting 285

A.4 Conclusion 285

References 286

Index 289

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)