Gel Electrophoresis: Nucleic Acids / Edition 1

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More About This Textbook


Through its clear presentation of the basic concepts, Gel Electrophoresis: Nucleic Acids breaks new ground by describing the principles of the technique without resorting to complicated protocols and recipes.

Incl. theory of nucleic acid electrophoresis, pulsed field, detection following electrophoretic separation, etc.

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Product Details

  • ISBN-13: 9781872748283
  • Publisher: Taylor & Francis
  • Publication date: 1/1/1996
  • Series: Introduction to Biotechniques Series
  • Edition number: 1
  • Pages: 192
  • Product dimensions: 6.14 (w) x 9.21 (h) x 0.42 (d)

Table of Contents

1 Introduction: the Variety and Forms of Nucleic Acids 1
2 The Theory of Nucleic Acid Electrophoresis 9
3 The Electrophoresis of Native and Denatured Nucleic Acids 23
4 The Choice of Format: Horizontal or Vertical, Agarose or Polyacrylamide? 31
5 The Detection of Nucleic Acids Following Electrophoretic Separation 49
6 Guide to Techniques and Applications 71
7 Nondenaturing Agarose Gel Electrophoresis 75
8 Denaturing Agarose Gel Electrophoresis 101
9 Pulsed Field Agarose Gel Electrophoresis 107
10 Nondenaturing Polyacrylamide Gel Electrophoresis 117
11 Denaturing Polyacrylamide Gel Electrophoresis 137
Appendix A: Glossary 167
Appendix B: Suppliers 171
Index 173
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