Gel Electrophoresis of Proteins and Nucleic Acids: Selected Techniques

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Editorial Reviews

Booknews
Identified in the preface as a third edition (with no further information except that "the first German edition...was written in 1968"), this handbook emphasizes both the theory and practice of electrophetic techniques. Seven chapter address: support media; gel electrophoresis of proteins and nucleic acids. isoelectric focusing, two-dimensional electrophoresis; component visualization; image handling procedures; and protocols and procedures. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9783110138962
  • Publisher: De Gruyter, Walter, Inc.
  • Publication date: 6/28/1994
  • Pages: 352
  • Product dimensions: 6.96 (w) x 9.66 (h) x 0.86 (d)

Table of Contents

Ch. 1 Support Media
Polyacrylamide 1
Agarose 16
Ch. 2 Gel Electrophoresis of Proteins and Nucleic Acids
Principles of Electrophoresis 26
Control of Joule Heat 29
Continuous zone (CZE) vs. Multiphasic (MZE) Electrophoresis 32
Moving Boundary Buffer Systems for Producing Narrow Starting Zones 35
CZE Electrophoresis 47
Molecular Weight Determination 52
Nucleic Acid separation 62
Ch. 3 Isoelectric Focusing
Isoelectric Focusing Procedures 93
Practical Application of Theory 100
PAGIF in Clinical Medicine 110
Trouble Shooting Guide 140
Ch. 4 Two-Dimensional Electrophoresis
Two-Dimensional Databases 187
Ch. 5 Component Visualization
Protein Stains 204
Silver Staining 213
Enzyme Visualization 224
Nucleic Acid Visualization 254
Ch. 6 Image Handling Procedures
Photography 273
Densitometry and Direct Image Processing 276
Ch. 7 Protocols and Procedures
Gel Casting 285
Agarose 296
DNA Probes 308
References 332
Abbreviations 335
Subject Index 339
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