Gene Cloning and DNA Analysis: An Introduction / Edition 5

Gene Cloning and DNA Analysis: An Introduction / Edition 5

by T. A. Brown
     
 

ISBN-10: 1405111216

ISBN-13: 9781405111218

Pub. Date: 03/28/2006

Publisher: Wiley

Explaining the significance, the principles, the techniques, and the applications of gene cloning and DNA analysis, this introduction assumes little prior knowledge on the part of the reader. Particular attention is given to the methods of sequencing genomes and analyzing this sequence. The applications to forensics science are highlighted. Approximately 250 color

Overview

Explaining the significance, the principles, the techniques, and the applications of gene cloning and DNA analysis, this introduction assumes little prior knowledge on the part of the reader. Particular attention is given to the methods of sequencing genomes and analyzing this sequence. The applications to forensics science are highlighted. Approximately 250 color illustrations are featured. Brown teaches biomolecular archeology at UNMIST.

Annotation c. Book News, Inc., Portland, OR (booknews.com)

Product Details

ISBN-13:
9781405111218
Publisher:
Wiley
Publication date:
03/28/2006
Edition description:
REV
Pages:
408
Product dimensions:
7.40(w) x 9.60(h) x 1.10(d)

Table of Contents

Preface to the Fourth Editionvii
Preface to the Third Editionix
Preface to the Second Editionx
Preface to the First Editionxii
Part 1The Basic Principles of Gene Cloning and DNA Analysis1
1Why Gene Cloning and DNA Analysis are Important3
2Vehicles for Gene Cloning: Plasmids and Bacteriophages14
3Purification of DNA from Living Cells28
4Manipulation of Purified DNA52
5Introduction of DNA into Living Cells85
6Cloning Vectors for E. coli104
7Cloning Vectors for Eukaryotes130
8How to Obtain a Clone of a Specific Gene156
9The Polymerase Chain Reaction179
Part 2The Applications of Gene Cloning and DNA Analysis in Research195
10Studying Gene Location and Structure197
11Studying Gene Expression and Function217
12Studying Genomes246
Part 3The Applications of Gene Cloning and DNA Analysis in Biotechnology269
13Production of Protein from Cloned Genes271
14Gene Cloning and DNA Analysis in Medicine293
15Gene Cloning and DNA Analysis in Agriculture313
16Gene Cloning and DNA Analysis in Forensic Science329
Glossary341
Index357

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